Tue Dec 13 2016 11:16:37 GMT+0100 (CET)2025-05-23 16:04:55
locus
BSU39020
BSU_39020
ec
outlinks
bsu
BSU39020
BSU_39020
aminos
MYFLLNLVGLIVIMAVVFLCSPQKKKIKWRPIITLIVLELLITWFMLGTKVGSWAIGKIGDFFTWLIACASDGIAFAFPSVMANETVDFFFSALLPIIFIVTFFDILTYFGILPWLIDKIGWVISKASRLPKLESFFSIQMMFLGNTEALAVIRQQLTVLSNNRLLTFGLMSMSSISGSIIGSYLSMVPATYVFTAIPLNCLNALIIANLLNPVHVPEDEDIIYTPPKEEKKDFFSTISNSMLVGMNMVIVILAMVIGYVALTSAVNGILGVFVHGLTIQTIFAYLFSPFAFLLGLPVHDAMYVAQLMGMKLATNEFVAMLDLKNNLKSLPPHTVAVATTFLTSFANFSTVGMIYGTYNSILDGEKSTVIGRNVWKLLVSGIAVSLLSAAIVGLFVW
[SW|Categories] containing this gene/protein
[SW|transporters/ other], [SW|biosynthesis/ acquisition of nucleotides], [SW|membrane proteins]
Gene
Coordinates on the chromosome (coding sequence)
4,005,752 -> 4,006,945
Gene
Phenotypes of a mutant
a ''[[protein|nupN]] [[protein|nupG]]'' double mutant is unable to utilize externally provided guanosine [Pubmed|21926227]
a ''[[gene|nupN]] [[gene|nupG]]'' double mutant is unable to utilize externally provided guanosine [Pubmed|21926227]
The protein
Protein family
SLC28A transporter family (according to Swiss-Prot)
concentrative nucleoside transporter (CNT) (TC 2.A.41) family (with [[protein|NupC]] and [[protein|YutK]], according to UniProt)
Expression and Regulation
Operon
''nupG
Expression and Regulation
Regulation
expression activated by glucose (4.3 fold) [Pubmed|12850135]
repressed by hypoxanthine and guanine, [[protein|PurR]]-independent, [[protein|G-box]][Pubmed|12923093]
Expression and Regulation
Regulatory mechanism
[[protein|G-box]]: transcriptional termination/ antitermination [SW|riboswitch] [Pubmed|12923093]
Biological materials
Mutant
CS219 (''[[protein|nupG]]''::''aphA3'', available in [SW|Colin Harwood]'s and [SW|Jörg Stülke]'s labs) [Pubmed|27197833]
MGNA-B725 (yxjA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1724 NBRP B. subtilis, Japan]
CS219 (''[[gene|nupG]]''::''aphA3'', available in [SW|Colin Harwood]'s and [SW|Jörg Stülke]'s labs) [Pubmed|27197833]
BKE39020 (Δ[[gene|nupG]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAGCAACTCCTATGA, downstream forward: _UP4_TAGAAACATTGAGCCATATC
BKK39020 (Δ[[gene|nupG]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAGCAACTCCTATGA, downstream forward: _UP4_TAGAAACATTGAGCCATATC
References
proteinLength
397
geneLength
1194
Gene
Coordinates
4,005,752 → 4,006,945
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|3TIJ] (from Vibrio cholerae, 25% identity) [pubmed|22407322]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]