Tue Dec 13 2016 11:16:37 GMT+0100 (CET)2025-05-23 21:02:04
locus
BSU39720
BSU_39720
product
2-keto-myo-inositol dehydratase,
dehydration of 2-keto-myo-inositol (2nd reaction)
2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)
outlinks
bsu
BSU39720
BSU_39720
aminos
MGKNEILWGIAPIGWRNDDMPEIGAGNTLQHLLSDIVVARFQGTEVGGFFPEPAILNKELKLRNLRIAGKWFSSFILRDGLGEAAKTFTLHCEYLQQVNADVAVVSEQTYSVQSLEKNVFTEKPHFTDDEWERLCEGLNHLGEIAAQHGLKLVYHHHLGTGVQTAEEVDRLMAGTDPAHVHLLYDTGHAYISDGDYMGMLEKHIGRIKHVHFKDARLNVMEQCRLEGQSFRQSFLKGMFTVPGDGCIDFREVYQLLLKHSYSGWIVIEAEQDPDVANPLEYALIARNYIDQQLLDLA
[SW|Categories] containing this gene/protein
[SW|utilization of specific carbon sources]
Gene
Coordinates on the chromosome (coding sequence)
4,078,173 -> 4,079,066
The protein
Catalyzed reaction/ biological activity
2,4,6/3,5-pentahydroxycyclohexanone = 3,5/4-trihydroxycyclohexa-1,2-dione + H2O (according to Swiss-Prot)
scyllo-inosose --> 3D-3,5/4-trihydroxycyclohexane-1,2-dione + H2O (according to UniProt)
The protein
Protein family
iolE/mocC family (according to Swiss-Prot)
IolE/MocC family (single member, according to UniProt)
Expression and Regulation
Operon
''[[protein|iolA]]-[[protein|iolB]]-[[protein|iolC]]-[[protein|iolD]]-[[protein|iolE]]-[[protein|iolF]]-[[protein|iolG]]-[[protein|iolH]]-[[protein|iolI]]-[[protein|iolJ]]'' [Pubmed|9226270]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|9226270]
Expression and Regulation
Regulation
repressed by glucose ([[protein|CcpA]]) [Pubmed|10666464]
induced by inositol ([[protein|IolR]]) [Pubmed|9887260,9226270]
Expression and Regulation
Regulatory mechanism
[[protein|IolR]]: transcription repression [Pubmed|9887260,9226270]
[[protein|CcpA]]: transcription repression [Pubmed|10666464]
Labs working on this gene/protein
[SW|Yasutaro Fujita], University of Fukuyama, Japan
[[Ken-ichi Yoshida]], Kobe University, Japan
proteinLength
297
geneLength
894
Gene
Coordinates
4,078,173 → 4,079,066
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-B774 (iolE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1773 NBRP B. subtilis, Japan]
BKE39720 (Δ[[gene|iolE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTGCCCATCTATGGTGCCT, downstream forward: _UP4_CTGGCTTAATGGGGAATGAA
BKK39720 (Δ[[gene|iolE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTGCCCATCTATGGTGCCT, downstream forward: _UP4_CTGGCTTAATGGGGAATGAA
labs
[SW|Yasutaro Fujita], University of Fukuyama, Japan
[[Ken-ichi Yoshida]], Kobe University, Japan