SubtiBank SubtiBank
Version comparison:

Sat Dec 10 2016 22:44:02 GMT+0100 (CET)2025-05-12 10:38:25

locus

BSU32530

BSU_32530

ec

outlinks

bsu

BSU32530

BSU_32530

aminos

MEKQKLSVKNSITDYIEWLAQYGASADGGVTRLLYTKEWMDAQLAVKTEMSSFGLETRFDDVGNVFGRLSGTQSPDEVIVTGSHIDTVINGGKYDGAYGVLAAMLALKQLKETYGAPKKTLEAVSLCEEEGSRFPMTYWGSGNMTGVFSEQDAKEPRDESGVSLQTAMHESGFGKGVFQSAYRTDISAFVELHIEQGKTLEMSGRDLGIVTSIAGQRRYLVTLEGECNHAGTTSMKWRKDPLAASSRIIHELLLRSDELPDELRLTCGKITAEPNVANVIPGRVQFSIDIRHQHQHVLEQFHQDMVALINGICLQKGIRAVIDEYMRIEPVPMDERLKAAAFETALENGFSCEEMVSGAGHDAQMIGRRYPACMLFVPSRGGVSHSPKEYTSARQLEIGVRALTDLLYKLAY

[SW|Categories] containing this gene/protein

[SW|utilization of nucleotides]

Gene

Coordinates on the chromosome (coding sequence)

3,342,433 -> 3,343,671

The protein

Protein family

peptidase M20 family (according to Swiss-Prot)

[SW|peptidase M20 family] (according to UniProt)

Expression and Regulation

Operon

''[[protein|pucF]]-[[protein|pucG]]'' [Pubmed|12029039]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|12029039]

Expression and Regulation

Regulation

induced in the presence of purine nucleotides (inducer: allantoin) ([[protein|PucR]]) [Pubmed|12029039]

Expression and Regulation

Regulatory mechanism

[[protein|PucR]]: transcription activation [Pubmed|12029039]

proteinLength

412

geneLength

1239

Gene

Coordinates

3,342,433 → 3,343,671

The protein

Catalyzed reaction/ biological activity

allantoate + 2 H+ + H2O --> (S)-2-ureidoglycine + CO2 + NH4+ (according to UniProt)

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-A943 (yurH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/943 NBRP B. subtilis, Japan]

BKE32530 (Δ[[gene|pucF]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTACTCCCCCCTCTGGC, downstream forward: _UP4_GCTTACTGATAAAGGAGGAA

BKK32530 (Δ[[gene|pucF]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTACTCCCCCCTCTGGC, downstream forward: _UP4_GCTTACTGATAAAGGAGGAA