SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:34 GMT+0100 (CET)2025-04-07 14:35:58

locus

BSU24620

BSU_24620

function

biofilm formation

[SW|biofilm formation]

ec

outlinks

bsu

BSU24620

BSU_24620

aminos

MGMKKKLSLGVASAALGLALVGGGTWAAFNDIKSKDATFASGTLDLSAKENSASVNLSNLKPGDKLTKDFQFENNGSLAIKEVLMALNYGDFKANGGSNTSPEDFLSQFEVTLLTVGKEGGNGYPKNIILDDANLKDLYLMSAKNDAAAAEKIKKQIDPKFLNASGKVNVATIDGKTAPEYDGVPKTPTDFDQVQMEIQFKDDKTKDEKGLMVQNKYQGNSIKLQFSFEATQWNGLTIKKDHTDKDGYVKENEKAHSEDKN

[SW|Categories] containing this gene/protein

[SW|biofilm formation]

Gene

Coordinates on the chromosome (coding sequence)

2,553,081 -> 2,553,866

Gene

Phenotypes of a mutant

altered cell death pattern in colonies [Pubmed|23012477]

altered cell death pattern in colonies [Pubmed|23012477]

increases membrane fluidity and cell death [pubmed|32313019]

The protein

Protein family

peptidase M73 family (according to Swiss-Prot)

peptidase M73 family (single member, according to UniProt)

The protein

[SW|Localization]

extracellular (signal peptide) [Pubmed|18957862], secretion requires [[protein|SipW]] [Pubmed|10049401]

membrane [Pubmed|18763711]

extracellular (signal peptide) [Pubmed|18957862], secretion requires [[protein|SipW]] [Pubmed|10049401]

The protein

[SW|Interactions]

forms fibers of variable length and 10-15 nm in width [Pubmed|20080671]

[[protein|TapA]]-[[protein|TasA]] [Pubmed|24488317]

[[protein|SipW]]-[[protein|TasA]] [Pubmed|10049401]

Expression and Regulation

Operon

''[[protein|tapA]]-[[protein|sipW]]-[[protein|tasA]]'' [Pubmed|16430695]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|16430695]

Expression and Regulation

Regulation

repressed by casamino acids [Pubmed|12107147]

repressed by [[protein|SinR]] [Pubmed|16430695]

repressed during logrithmic growth ([[protein|AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10464223,17720793 PubMed]

Expression and Regulation

Regulatory mechanism

[[protein|AbrB]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10464223,17720793 PubMed]

[[protein|SinR]]: transcription repression [Pubmed|16430695]

[[protein|RemA]]: transcription activation [Pubmed|23646920]

Expression and Regulation

Additional information

induction by sequestration of [[protein|SinR]] by [[protein|SinI]] or [[protein|SlrA]] [PubMed|15661000,19788541] or by SlrR [PubMed|20351052]

the '[[protein|tapA]]-[[protein|sipW]]-[[protein|tasA]]' operon is not expressed in a '[[protein|ymdB]]' mutant [PubMed|21856853]

the amount of the mRNA is substantially decreased upon depletion of [[Rny|RNase Y]] (this is likely due to the increased stability of the '[[protein|sinR]]' mRNA) [PubMed|21815947]

the mRNA is cleaved by [[protein|Rnc|RNase III]] [PubMed|26883633]

References

Reviews

20735481, 20351052, 21488983, 24988880, 25907113, 26418850

20735481, 20351052, 21488983, 24988880, 25907113, 26418850, 28783117, 30098341

References

Original publications

23352144, 23352134, 23632024, 23012477, 21815947, 21856853, 18047568, 16430695, 16430696, 10368135, 18430133, 18647168, 10464223, 17720793, 18957862, 12107147, 18763711, 20080671, 10049401, 23646920, 24488317, 25894589

23352144, 23352134, 23632024, 23012477, 21815947, 21856853, 18047568, 16430695, 16430696, 10368135, 18430133, 18647168, 10464223, 17720793, 18957862, 12107147, 20080671, 10049401, 23646920, 24488317, 25894589, 26883633, 29531041, 29565118, 29590605, 29802781, 29887307, 29921978, 30297741, 30833350, 31370706, 32313019, 32430292, 32491277, 32753507, 32779018, 33033811

proteinLength

261

geneLength

786

Gene

Coordinates

2,553,081 → 2,553,866

The protein

Paralogous protein(s)

[[this]]

The protein

Structure

[PDB|5OF1] [pubmed|29531041]

[PDB|5OF2] [pubmed|29531041]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-C449 (yqhF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2447 NBRP B. subtilis, Japan]

BKE24620 (Δ[[gene|tasA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTAAGCTCCCCTTTTA, downstream forward: _UP4_TAATAACAGCAAAAAAAAGA

BKK24620 (Δ[[gene|tasA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTAAGCTCCCCTTTTA, downstream forward: _UP4_TAATAACAGCAAAAAAAAGA