2017-8-11 16:0:32025-05-14 07:26:49

_ec

locus

BSU19210

BSU_19210

geneLength

861

864

outlinks

bsu

BSU19210

BSU_19210

The protein

[SW|Domains]

contains two N-acetylglucosamine-polymer-binding [SW|LysM domain]s [Pubmed|18430080]

2 [SW|LysM domain]s (aa 26-69, aa 78-121) (according to UniProt)

Biological materials

Mutant

MGNA-A321 (yocH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/321 NBRP B. subtilis, Japan]

AH023 knock-out mutant (kan), available in [SW|Kevin Devine]'s and [SW|Jörg Stülke]'s labs [Pubmed|17581128]

BKE19210 (''[[gene|yocH]]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]

MGNA-A321 (yocH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/321 NBRP B. subtilis, Japan]

AH023 knock-out mutant (kan), available in [SW|Kevin Devine]'s and [SW|Jörg Stülke]'s labs [Pubmed|17581128]

BKE19210 (''[[gene|yocH]]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]

BKE19210 (Δ[[gene|yocH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGTAAATCCTCCCTTG, downstream forward: _UP4_TAATAATATAGATTGTGATA

BKK19210 (Δ[[gene|yocH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGTAAATCCTCCCTTG, downstream forward: _UP4_TAATAATATAGATTGTGATA

Biological materials

Expression vector

pGP1691, expression of ''yocH''-Strep in ''B. subtilis'', in [SW|pGP382], available in [SW|Jörg Stülke]'s lab

pGP1693, expression of ''yocH''-Strep in ''E. coli'', in [SW|pGP574], available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

[SW|Kevin Devine]

References

Original publications

14651647, 15101989, 18957862, 12950927, 20059685, 20070526, 23199363, 17581128

14651647, 15101989, 18957862, 12950927, 20059685, 20070526, 23199363, 17581128, 31808740, 26163297

The protein

Paralogous protein(s)

[[this]]

The protein

Structure

[PDB|4WLK] ([[protein|YuiC]], corresponds to aa 155 ... 286 of [[protein|YocH]], 34% identity) [pubmed|26163297]

Biological materials

Expression vectors

pGP1691, expression of ''yocH''-Strep in ''B. subtilis'', in [SW|pGP382], available in [SW|Jörg Stülke]'s lab

pGP1693, expression of ''yocH''-Strep in ''E. coli'', in [SW|pGP574], available in [SW|Jörg Stülke]'s lab

labs

[SW|Kevin Devine]