2018-07-26 12:52:092025-05-27 18:29:42
locus
BSU12860
BSU_12860
geneLength
1314
1317
ec
outlinks
bsu
BSU12860
BSU_12860
The protein
[SW|Localization]
membrane
cell membrane (according to UniProt)
Biological materials
Mutant
MGNA-A736 (ykbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/736 NBRP B. subtilis, Japan]
BKE12860 (Δ[[gene|steT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTTAACCTCCTATG, downstream forward: _UP4_TGATAAAACGGTTCCCTTGT
BKK12860 (Δ[[gene|steT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTTAACCTCCTATG, downstream forward: _UP4_TGATAAAACGGTTCCCTTGT
GP2378 ∆''[[gene|steT]]'' ::''cat'', available in [SW|Jörg Stülke]'s lab [pubmed|32743959]
GP2945 ∆''[[gene|steT]]'' ::''kan'', available in [SW|Jörg Stülke]'s lab
MGNA-A736 (ykbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/736 NBRP B. subtilis, Japan]
BKE12860 (Δ[[gene|steT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTTAACCTCCTATG, downstream forward: _UP4_TGATAAAACGGTTCCCTTGT
BKK12860 (Δ[[gene|steT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTTAACCTCCTATG, downstream forward: _UP4_TGATAAAACGGTTCCCTTGT
References
The protein
Protein family
[SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|6F34] (from Geobacillus kaustophilus, 25% identity) [pubmed|29416041]
Biological materials
Expression vector
pGP2281: expression of ''steT'' by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab
Biological materials
lacZ fusion
pGP2275 (in [SW|pAC5]) (GP2962), available in [SW|Jörg Stülke]'s lab