locus

BSU12860

BSU_12860

geneLength

1314

1317

outlinks

bsu

BSU12860

BSU_12860

The protein

[SW|Localization]

membrane

cell membrane (according to UniProt)

Biological materials

Mutant

MGNA-A736 (ykbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/736 NBRP B. subtilis, Japan]

BKE12860 (Δ[[gene|steT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTTAACCTCCTATG, downstream forward: _UP4_TGATAAAACGGTTCCCTTGT

BKK12860 (Δ[[gene|steT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTTAACCTCCTATG, downstream forward: _UP4_TGATAAAACGGTTCCCTTGT

GP2378 ∆''[[gene|steT]]'' ::''cat'', available in [SW|Jörg Stülke]'s lab [pubmed|32743959]

GP2945 ∆''[[gene|steT]]'' ::''kan'', available in [SW|Jörg Stülke]'s lab

MGNA-A736 (ykbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/736 NBRP B. subtilis, Japan]

BKE12860 (Δ[[gene|steT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTTAACCTCCTATG, downstream forward: _UP4_TGATAAAACGGTTCCCTTGT

BKK12860 (Δ[[gene|steT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTTAACCTCCTATG, downstream forward: _UP4_TGATAAAACGGTTCCCTTGT

The protein

Protein family

[SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)

Biological materials

Expression vector

pGP2281: expression of ''steT'' by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

Biological materials

lacZ fusion

pGP2275 (in [SW|pAC5]) (GP2962), available in [SW|Jörg Stülke]'s lab