SubtiBank SubtiBank
Version comparison:

Mon Aug 31 2015 13:54:16 GMT+0200 (CEST)2025-05-12 11:30:29

locus

BSU15850

BSU_15850

outlinks

bsu

BSU15850

BSU_15850

syns

yloW, sdaA

[SW|Categories] containing this gene/protein

[SW|utilization of amino acids]

The protein

Catalyzed reaction/ biological activity

L-serine = pyruvate + NH3 (according to Swiss-Prot)

L-serine --> NH4+ + pyruvate (according to UniProt)

The protein

Protein family

ACT domain (according to Swiss-Prot)

iron-sulfur dependent L-serine dehydratase family (with [[protein|SdaAA]], according to UniProt)

The protein

[SW|Interactions]

[SW|SdaAA]-[SW|SdaAB] [Pubmed|22686449]

Expression and Regulation

Operon

''[SW|sdaAB]-[SW|sdaAA]'' [Pubmed|22383849]

References

22383849, 22686449, 24161940

22383849, 22686449, 24161940, 32743959

proteinLength

220

geneLength

663

Gene

Coordinates

1,658,242 → 1,658,904

Gene

Phenotypes of a mutant

overexpression of [[gene|sdaAB]]-[[gene|sdaAA]] results in resistance to serine [pubmed|32743959]

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|ACT domain] (aa 148-220) (according to UniProt)

The protein

[SW|Cofactors]

Fe-S cluster [pubmed|29292548]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE15850 (Δ[[gene|sdaAB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTATTCCTCCTTATGA, downstream forward: _UP4_TAGCGAAAAGGGTCAGGAGG

BKK15850 (Δ[[gene|sdaAB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTATTCCTCCTTATGA, downstream forward: _UP4_TAGCGAAAAGGGTCAGGAGG

Biological materials

lacZ fusion

pGP2275 (in [SW|pAC5]) (GP2967), available in [SW|Jörg Stülke]'s lab [pubmed|32743959]