SubtiBank SubtiBank
Version comparison:

Mon Aug 31 2015 13:54:20 GMT+0200 (CEST)2025-05-22 14:39:36

locus

BSU16400

BSU_16400

ec

outlinks

bsu

BSU16400

BSU_16400

[SW|Categories] containing this gene/protein

[SW|motility and chemotaxis], [SW|membrane proteins]

Gene

Coordinates on the chromosome (coding sequence)

1,709,747 -> 1,710,847

The protein

Protein family

GTP-binding [[protein|SRP]] family (according to Swiss-Prot)

GTP-binding SRP family (with [[protein|Ffh]] and [[protein|FtsY]], according to UniProt)

The protein

Structure

[PDB|2PX3] (complexed with GTP-Mg2) [Pubmed|17699634]

[PDB|3SYN] (complex with its activator [[protein|YlxH]]) [Pubmed|22056770]

[PDB|2PX3] (complexed with GTP-Mg2+) [Pubmed|17699634]

[PDB|3SYN] (complex with its activator [[protein|FlhG]]) [Pubmed|22056770]

The protein

[SW|Localization]

cell membrane (according to Swiss-Prot)

cell membrane (according to UniProt)

The protein

[SW|Interactions]

[[protein|FlhG]]-[[protein|FlhF]] ([[protein|FlhG]] binds the GTP-bound homodimer of [[protein|FlhF]]) [Pubmed|22056770]

Expression and Regulation

Operon

part of the [[fla-che operon]]

''[[protein|flgB]]-[[protein|flgC]]-[[protein|fliE]]-[[protein|fliF]]-[[protein|fliG]]-[[protein|fliH]]-[[protein|fliI]]-[[protein|fliJ]]-[[protein|ylxF]]-[[protein|fliK]]-[[protein|flgD]]-[[protein|flgE]]-[[protein|fliL]]-[[protein|fliM]]-[[protein|fliY]]-[[protein|cheY]]-[[protein|fliZ]]-[[protein|fliP]]-[[protein|fliQ]]-[[protein|fliR]]-[[protein|flhB]]-[[protein|flhA]]-[[protein|flhF]]-[[protein|flhG]]-[[protein|cheB]]-[[protein|cheA]]-[[protein|cheW]]-[[protein|cheC]]-[[protein|cheD]]-[[protein|sigD]]-[[protein|swrB]]'' [Pubmed|9657996,8157612,15175317]

''[[protein|ylxF]]-[[protein|fliK]]-[[protein|flgD]]-[[protein|flgE]]-[[protein|fliL]]-[[protein|fliM]]-[[protein|fliY]]-[[protein|cheY]]-[[protein|fliZ]]-[[protein|fliP]]-[[protein|fliQ]]-[[protein|fliR]]-[[protein|flhB]]-[[protein|flhA]]-[[protein|flhF]]-[[protein|flhG]]-[[protein|cheB]]-[[protein|cheA]]-[[protein|cheW]]-[[protein|cheC]]-[[protein|cheD]]-[[protein|sigD]]-[[protein|swrB]]'' [Pubmed|20233303]

Expression and Regulation

[SW|Sigma factor]

''[[protein|flgB]]'': [[protein|SigA]] [Pubmed|9657996], [[protein|SigD]] [Pubmed|9657996]

''[[protein|ylxF]]'': [[protein|SigD]] [Pubmed|20233303]

Expression and Regulation

Regulation

see [[fla-che operon]]

Expression and Regulation

Regulatory mechanism

see [[fla-che operon]]

Biological materials

Mutant

1A925 ( ''flhF''::''cat''), [Pubmed|15317803], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A925&Search=1A925 BGSC]

1A925 ( ''flhF''::''cat''), [Pubmed|15317803], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A925&Search=1A925 BGSC]

BKE16400 (Δ[[gene|flhF]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAATATCCACCACTCC, downstream forward: _UP4_CTGTGCAGATGAACAGATAT

BKK16400 (Δ[[gene|flhF]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAATATCCACCACTCC, downstream forward: _UP4_CTGTGCAGATGAACAGATAT

References

Original Publications

1447978, 17699634, 17850253, 15317803, 14651647, 9657996, 8157612, 15175317, 22056770, 23190039, 24386445

1447978, 17699634, 17850253, 15317803, 14651647, 9657996, 8157612, 15175317, 22056770, 23190039, 24386445, 27935957

proteinLength

366

geneLength

1101

Gene

Coordinates

1,709,747 → 1,710,847

Gene

Phenotypes of a mutant

inactivation of ''[[gene|flhF]]'' confers resistance to high concentrations of Zn(II) [Pubmed|27935957]

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]