2017-09-16 17:38:502025-05-27 00:47:08

description

single-strand DNA-specific exonuclease

single-strand DNA-specific exonuclease, acts together with [[protein|RecQ]] in replication fork maintenance

locus

BSU27620

BSU_27620

geneLength

2358

2361

function

DNA repair

replication fork maintenance

outlinks

bsu

BSU27620

BSU_27620

Gene

Phenotypes of a mutant

''[[gene|recO]] [[gene|recJ]]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]

suppression of lethality of [[gene|pcrA]] inactivation [pubmed|32793628]

The protein

Protein family

recJ family (according to Swiss-Prot)

RecJ family (with [[protein|YorK]], according to UniProt)

The protein

Paralogous protein(s)

[[protein|YorK]]

[[this]]

The protein

[SW|Localization]

cytoplasm (according to Swiss-Prot)

associated with the [SW|replisome] [pubmed|21170359]

bound to replication forks, but also assembles at many sites on the nucleoid upon DNA damage induction [pubmed|30401797]

Biological materials

Mutant

MGNA-B516 (yrvE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1515 NBRP B. subtilis, Japan]

GP895 (''[[gene|recJ]]''::''kan''), available in [SW|Jörg Stülke]'s lab

BKE27620 (''[[gene|recJ]]''::''erm'', available in the BGSC, in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s labs) [pubmed|28189581]

BKE27620 (Δ[[gene|recJ]]::erm trpC2), available at [http://www.bgsc.org/getdetail.php?bgscid=BSU27620 BGSC], upstream reverse:

_UP1_CATAAATTCACCCCTCAACC

, downstream forward:

_UP4_AGTACGAGGAGGACATAAAA

BKK27620 (Δ[[gene|recJ]]::kan trpC2), available at [http://www.bgsc.org/getdetail.php?bgscid=BSU27620 BGSC], upstream reverse:

_UP1_CATAAATTCACCCCTCAACC

, downstream forward:

_UP4_AGTACGAGGAGGACATAAAA

MGNA-B516 (yrvE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1515 NBRP B. subtilis, Japan]

GP895 (''[[gene|recJ]]''::''kan''), available in [SW|Jörg Stülke]'s lab

BKE27620 (''[[gene|recJ]]''::''erm'', available in the BGSC, in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s labs) [pubmed|28189581]

BKE27620 (Δ[[gene|recJ]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAATTCACCCCTCAACC, downstream forward: _UP4_AGTACGAGGAGGACATAAAA

BKK27620 (Δ[[gene|recJ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAATTCACCCCTCAACC, downstream forward: _UP4_AGTACGAGGAGGACATAAAA

References

Reviews

22933559

22933559, 32286623

References

Original publications

15241682, 11948165, 10498723, 21170359, 21205011, 26001966

15241682, 11948165, 10498723, 21170359, 21205011, 26001966, 27058167, 30401797, 32793628

The protein

[SW|Domains]

[SW|DHH-DHHA1 domain]

The protein

[SW|Cofactors]

Mn²⁺ [pubmed|27058167]

The protein

Structure

[PDB|5F54] (from Deinococcus radiodurans, the [SW|DHH-DHHA1 domain], 32% identity) [pubmed|27058167]