Mon Jan 02 2017 11:46:46 GMT+0100 (CET)2025-05-06 01:51:30
locus
BSU13930
BSU_13930
ec
outlinks
bsu
BSU13930
BSU_13930
[SW|Categories] containing this gene/protein
[SW|DNA repair/ recombination], [SW|sporulation proteins]
Gene
Coordinates on the chromosome (coding sequence)
1,461,770 -> 1,462,798
The protein
Catalyzed reaction/ biological activity
repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase
repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine in DNA --> thymidine dimer in DNA (according to UniProt)
The protein
[SW|Cofactors]
contains a [4Fe-4S] cluster, uses the [4Fe-4S] cluster to reduce SAM
Fe-S cluster [pubmed|29292548]
uses the [4Fe-4S] cluster to reduce SAM
Expression and Regulation
Operon
''[[protein|splA]]-[[protein|splB]]'' [Pubmed|8021181]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigG]] [Pubmed|15699190,8021181]
Expression and Regulation
Regulation
expressed during sporulation in the forespore ([[protein|SigG]]) [Pubmed|16497325]
Expression and Regulation
Regulatory mechanism
negative autoregulation by [[protein|SplA]] [Pubmed|8021181]
Biological materials
Mutant
BP130 (del ''splB''::''spc''), available in [SW|Fabian Commichau]'s lab
BP130 (Δ''splB''::''spc''), available in [SW|Fabian Commichau]'s and [SW|Jörg Stülke]'s labs [pubmed|30054368]
BKE13930 (Δ[[gene|splB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGCCACATCCTTTTC, downstream forward: _UP4_TTCACTTAAACGGGCTGTTG
BKK13930 (Δ[[gene|splB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGCCACATCCTTTTC, downstream forward: _UP4_TTCACTTAAACGGGCTGTTG
References
Reviews
References
Original publications
proteinLength
342
geneLength
1029
Gene
Coordinates
1,461,770 → 1,462,798
Gene
Phenotypes of a mutant
sensitive to blue light-induced DNA damage [pubmed|30054368]
The protein
Protein family
[SW|Radical SAM superfamily] (according to UniProt)
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]