SubtiBank SubtiBank
Version comparison:

Mon Jan 02 2017 11:46:46 GMT+0100 (CET)2025-05-06 01:51:30

locus

BSU13930

BSU_13930

ec

outlinks

bsu

BSU13930

BSU_13930

[SW|Categories] containing this gene/protein

[SW|DNA repair/ recombination], [SW|sporulation proteins]

Gene

Coordinates on the chromosome (coding sequence)

1,461,770 -> 1,462,798

The protein

Catalyzed reaction/ biological activity

repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase

repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase

(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine in DNA --> thymidine dimer in DNA (according to UniProt)

The protein

[SW|Cofactors]

contains a [4Fe-4S] cluster, uses the [4Fe-4S] cluster to reduce SAM

Fe-S cluster [pubmed|29292548]

uses the [4Fe-4S] cluster to reduce SAM

Expression and Regulation

Operon

''[[protein|splA]]-[[protein|splB]]'' [Pubmed|8021181]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigG]] [Pubmed|15699190,8021181]

Expression and Regulation

Regulation

expressed during sporulation in the forespore ([[protein|SigG]]) [Pubmed|16497325]

Expression and Regulation

Regulatory mechanism

negative autoregulation by [[protein|SplA]] [Pubmed|8021181]

Biological materials

Mutant

BP130 (del ''splB''::''spc''), available in [SW|Fabian Commichau]'s lab

BP130 (Δ''splB''::''spc''), available in [SW|Fabian Commichau]'s and [SW|Jörg Stülke]'s labs [pubmed|30054368]

BKE13930 (Δ[[gene|splB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGCCACATCCTTTTC, downstream forward: _UP4_TTCACTTAAACGGGCTGTTG

BKK13930 (Δ[[gene|splB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGCCACATCCTTTTC, downstream forward: _UP4_TTCACTTAAACGGGCTGTTG

References

Reviews

23164663, 25477522, 22197590

23164663, 25477522, 22197590, 26265564, 28027411

References

Original publications

15699190, 8021181, 21671623, 22197590, 23607538, 23799365, 22761404, 28027411, 16829676, 11470912

15699190, 8021181, 21671623, 22197590, 23607538, 23799365, 22761404, 16829676, 11470912, 22906093, 16829680, 11902862, 9733691, 28401144, 30054368

proteinLength

342

geneLength

1029

Gene

Coordinates

1,461,770 → 1,462,798

Gene

Phenotypes of a mutant

sensitive to blue light-induced DNA damage [pubmed|30054368]

The protein

Protein family

[SW|Radical SAM superfamily] (according to UniProt)

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]