SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:31 GMT+0100 (CET)2025-05-15 20:54:37

locus

BSU08630

BSU_08630

outlinks

bsu

BSU08630

BSU_08630

aminos

MNVLEDKLKQKDIQQFRDDLISWFEREQRVLPWREDQDPYKVWVSEVMLQQTRVETVIPYFLRFVEQFPTVEALADADEEKVLKAWEGLGYYSRVRNLQSAVKEVKQEYGGIVPPDEKDFGGLKGVGPYTKGAVLSIAYNKPIPAVDGNVMRVMSRILSIWDDIAKPKTRTIFEDAIRAFISKEKPSEFNQGLMELGALICTPKSPSCLLCPVQQHCSAFEEGTERELPVKSKKKKPGIKTMAAIVLTDEDGQVYIHKRPSKGLLANLWEFPNLETQKGIKTEREQLIAFLENEYGIQADISDLQGVVEHVFTHLVWNISVFFGKVKQVSDTSKLKKVTKEELEQFAFPVSHQKIWKMAGEAAAISAAP

[SW|Categories] containing this gene/protein

[SW|DNA repair/ recombination]

Gene

Coordinates on the chromosome (coding sequence)

935,656 -> 936,765

The protein

Catalyzed reaction/ biological activity

releases adenines from 8-oxo-G:A mismatches

releases adenines from 8-oxo-G:A mismatches

Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site (according to UniProt)

Expression and Regulation

Operon

''[[protein|mutY]]-[[protein|fabL]]-[[protein|sspE]]'' [Pubmed|10463184]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|10463184]

[[protein|SigG]] [Pubmed|1900507]

Expression and Regulation

Regulation

expression is constitutive throughout growth [Pubmed|20971907]

expression is fivefold increased in a ''[[protein|mutS]]-[[protein|mutL]]'' mutant [Pubmed|20971907]

[[protein|YfhP]] may act as a negative transcription regulator of the ''[[protein|mutY]]-[[protein|fabL]]-[[protein|sspE]]'' operon during exponential growth [Pubmed|10463184]

References

Original publications

10463184, 19011023, 20971907, 24973075, 25326311, 25995449, 27399782

10463184, 19011023, 20971907, 24973075, 25326311, 25995449, 27399782, 30691388

proteinLength

369

geneLength

1110

Gene

Coordinates

935,656 → 936,765

The protein

Protein family

Nth/MutY family (with [[protein|Nth]], according to UniProt)

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

HhH domain (aa 108-137) (according to UniProt)

The protein

[SW|Cofactors]

Fe-S cluster [pubmed|29292548]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-C322 (yfhQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2320 NBRP B. subtilis, Japan]

BKE08630 (Δ[[gene|mutY]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTGTTTCTCCCCAGT, downstream forward: _UP4_ATCTCGGCTGCTCCGTAAAC

BKK08630 (Δ[[gene|mutY]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTGTTTCTCCCCAGT, downstream forward: _UP4_ATCTCGGCTGCTCCGTAAAC