Tue Dec 13 2016 11:16:33 GMT+0100 (CET)2025-05-28 01:26:55
locus
BSU16130
BSU_16130
outlinks
bsu
BSU16130
BSU_16130
aminos
5812..7119BacillussubtilissubtilisMNQQTVNVIGAGLAGSEAAWQLAKRGIQVKLYEMRPVKQTPAHHTDKFAELVCSNSLRSNTLANAVGVLKEEMRALDSAIIAAADECSVPAGGALAVDRHEFAASVTNRVKNHPNVTVINEEVTEIPEGPTIIATGPLTSESLSAQLKELTGEDYLYFYDAAAPIVEKDSLDMDKVYLKSRYDKGEAAYLNCPMTEEEFDRFHEALTSAETVPLKEFEKEIFFEGCMPIEVMAKRGKKTMLFGPMKPVGLEHPVTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGDQKEVLKLIPGLENVEIVRYGVMHRNTFINSPSLLKPTYQFKNRSDLFFAGQMTGVEGYVESAASGLVAGINAAKLVLGEELVIFPQETAIGSMAHYITTTNQKNFQPMNANFGLLKELPVKIKNKKERNEQYANRAIETIQTISKTI
[SW|Categories] containing this gene/protein
[SW|translation]
Gene
Coordinates on the chromosome (coding sequence)
1,685,812 -> 1,687,119
The protein
Catalyzed reaction/ biological activity
catalyzes the folate-dependent C(5)-methylation of uridine at position 54 in the TpsiC loop of tRNA
catalyzes the folate-dependent C(5)-methylation of uridine at position 54 in the TpsiC loop of tRNA
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + FADH2 + uridine54 in tRNA --> (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine54 in tRNA + FAD + H+ (according to UniProt)
The protein
Protein family
TrmFO subfamily (according to Swiss-Prot)
MnmG family (with [[protein|GidA]], according to UniProt)
References
proteinLength
435
geneLength
1308
Gene
Coordinates
1,685,812 → 1,687,119
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-B147 (gid::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1146 NBRP B. subtilis, Japan]
BKE16130 (Δ[[gene|trmFO]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTCACATCTCCTAGT, downstream forward: _UP4_TAGGTATTGATTGCAACCTG
BKK16130 (Δ[[gene|trmFO]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTCACATCTCCTAGT, downstream forward: _UP4_TAGGTATTGATTGCAACCTG