SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:33 GMT+0100 (CET)2025-05-28 01:26:55

locus

BSU16130

BSU_16130

outlinks

bsu

BSU16130

BSU_16130

aminos

5812..7119BacillussubtilissubtilisMNQQTVNVIGAGLAGSEAAWQLAKRGIQVKLYEMRPVKQTPAHHTDKFAELVCSNSLRSNTLANAVGVLKEEMRALDSAIIAAADECSVPAGGALAVDRHEFAASVTNRVKNHPNVTVINEEVTEIPEGPTIIATGPLTSESLSAQLKELTGEDYLYFYDAAAPIVEKDSLDMDKVYLKSRYDKGEAAYLNCPMTEEEFDRFHEALTSAETVPLKEFEKEIFFEGCMPIEVMAKRGKKTMLFGPMKPVGLEHPVTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGDQKEVLKLIPGLENVEIVRYGVMHRNTFINSPSLLKPTYQFKNRSDLFFAGQMTGVEGYVESAASGLVAGINAAKLVLGEELVIFPQETAIGSMAHYITTTNQKNFQPMNANFGLLKELPVKIKNKKERNEQYANRAIETIQTISKTI

[SW|Categories] containing this gene/protein

[SW|translation]

Gene

Coordinates on the chromosome (coding sequence)

1,685,812 -> 1,687,119

The protein

Catalyzed reaction/ biological activity

catalyzes the folate-dependent C(5)-methylation of uridine at position 54 in the TpsiC loop of tRNA

catalyzes the folate-dependent C(5)-methylation of uridine at position 54 in the TpsiC loop of tRNA

(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + FADH2 + uridine54 in tRNA --> (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine54 in tRNA + FAD + H+ (according to UniProt)

The protein

Protein family

TrmFO subfamily (according to Swiss-Prot)

MnmG family (with [[protein|GidA]], according to UniProt)

References

16027442, 20412857, 21846722, 24228791, 21561081, 23157377, 23095745, 19416846, 24228791

16027442, 20412857, 21846722, 24228791, 21561081, 23157377, 23095745, 19416846, 24228791, 30968076

proteinLength

435

geneLength

1308

Gene

Coordinates

1,685,812 → 1,687,119

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-B147 (gid::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1146 NBRP B. subtilis, Japan]

BKE16130 (Δ[[gene|trmFO]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTCACATCTCCTAGT, downstream forward: _UP4_TAGGTATTGATTGCAACCTG

BKK16130 (Δ[[gene|trmFO]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTCACATCTCCTAGT, downstream forward: _UP4_TAGGTATTGATTGCAACCTG