SubtiBank SubtiBank
Version comparison:

2017-8-11 16:0:32025-05-30 13:49:32

locus

BSU13410

BSU_13410

geneLength

933

936

ec

outlinks

bsu

BSU13410

BSU_13410

Gene

Phenotypes of a mutant

sensitivity to ionizing radiation in the stationary phase [Pubmed|12215643]

sensitivity of spores to several DNA-damaging treatments known to cause double strand breaks, such as UV-ray, X-ray, ultrahigh vacuum and wet heat [Pubmed|16497325,17293412]

a ''[[gene|ykoV]]-[[gene|ligD]]'' double mutant is sensitive to radiation [Pubmed|24123749]

sensitivity to ionizing radiation in the stationary phase [Pubmed|12215643]

sensitivity of spores to several DNA-damaging treatments known to cause double strand breaks, such as UV-ray, X-ray, ultrahigh vacuum and wet heat [Pubmed|16497325,17293412]

a ''[[gene|ykoV]]-[[gene|ligD]]'' double mutant is sensitive to radiation [Pubmed|24123749]

reduced resistance towards electron beams [pubmed|31948638]

The protein

Catalyzed reaction/ biological activity

stimulation of [[protein|LigD]] non-homologous end-joining activity [Pubmed|23691176,26961308]

stimulation of [[protein|LigD]] non-homologous end-joining activity [Pubmed|23691176,26961308]

binds at the DNA double-strand breaks and recruits the ligase [[protein|LigD ]]to seal the break [pubmed|15778718,16518468]

Biological materials

Mutant

MGNA-A779 (ykoV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/779 NBRP B. subtilis, Japan]

BP141 (''[[gene|ykoV]]-[[gene|ligD]]''::''kan'') available in [SW|Fabian Commichau]'s lab

BP142 (''[[gene|dgcW]]-[[gene|ykoV]]-[[gene|ligD]]''::''kan'') available in [SW|Fabian Commichau]'s lab

MGNA-A779 (ykoV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/779 NBRP B. subtilis, Japan]

BP809 (Δ[[gene|ykoV]]::''cat'') available in [SW|Juan Alonso]'s and [SW|Jörg Stülke]'s labs [pubmed|16780573]

BP141 (Δ''[[gene|ykoV]]-[[gene|ligD]]''::''kan'') available in [SW|Fabian Commichau]'s and [SW|Jörg Stülke]'s labs [pubmed|30863384]

BP142 (Δ''[[gene|dgcW]]-[[gene|ykoV]]-[[gene|ligD]]''::''kan'') available in [SW|Fabian Commichau]'s lab

BKE13410 (Δ[[gene|ykoV]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTAGTAACTTATGGG, downstream forward: _UP4_AAAGCCTCCGGCACATCATA

BKK13410 (Δ[[gene|ykoV]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTAGTAACTTATGGG, downstream forward: _UP4_AAAGCCTCCGGCACATCATA

References

Reviews

11591342, 11719239, 17938628, 22933559

11591342, 11719239, 17938628, 22933559, 30746801

References

Original publications

16497325, 11483577, 23691176, 12215643, 17293412, 24123749, 25355514, 26961308

16497325, 11483577, 23691176, 12215643, 17293412, 24123749, 25355514, 26961308, 15778718, 16518468, 29234047, 30976810, 31948638

The protein

Protein family

prokaryotic Ku family (single member, according to UniProt)

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

KU domain (aa 26-210) (according to UniProt)