SubtiBank SubtiBank
Version comparison:

2017-8-11 16:0:32025-05-16 21:22:57

description

Modulator of CcpN activity

Modulator of [[protein|CcpN]] activity, similar to phosphoenolpyruvate synthetase regulatory protein

locus

BSU25240

BSU_25240

geneLength

810

813

function

inhibits CcpN activity

inhibits [[protein|CcpN]] activity

product

modulator of CcpN activity

modulator of [[protein|CcpN]] activity

outlinks

bsu

BSU25240

BSU_25240

The protein

Catalyzed reaction/ biological activity

negative effector of [[protein|CcpN]] activity [Pubmed|15720552]

negative effector of [[protein|CcpN]] activity [Pubmed|15720552]

ADP + Nτ-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase] --> AMP + H+ + Nτ-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase] (according to UniProt)

H+ + Nτ-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase] + phosphate --> diphosphate + Nτ-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase] (according to UniProt)

The protein

Protein family

pyruvate, phosphate dikinase regulatory protein family (according to Swiss-Prot) pyruvate, phosphate dikinase regulatory protein family

pyruvate, phosphate/water dikinase regulatory protein family (single member, according to UniProt)

Biological materials

Mutant

MGNA-C492 (yqfL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2490 NBRP B. subtilis, Japan]

MGNA-C492 (yqfL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2490 NBRP B. subtilis, Japan]

BKE25240 (Δ[[gene|yqfL]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATCCCCCCGTTAGT, downstream forward: _UP4_TAACTCAGGACGCTCTATCC

BKK25240 (Δ[[gene|yqfL]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATCCCCCCGTTAGT, downstream forward: _UP4_TAACTCAGGACGCTCTATCC

Labs working on this gene/protein

[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

15720552

15720552, 26620526, 20044937

The protein

Paralogous protein(s)

[[this]]

The protein

Structure

[PDB|5D0N] (from maize, 39% identity) [pubmed|26620526]

labs

[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France