2017-8-11 16:0:32025-05-16 21:22:57
description
Modulator of CcpN activity
Modulator of [[protein|CcpN]] activity, similar to phosphoenolpyruvate synthetase regulatory protein
locus
BSU25240
BSU_25240
geneLength
810
813
function
inhibits CcpN activity
inhibits [[protein|CcpN]] activity
product
modulator of CcpN activity
modulator of [[protein|CcpN]] activity
outlinks
bsu
BSU25240
BSU_25240
The protein
Catalyzed reaction/ biological activity
negative effector of [[protein|CcpN]] activity [Pubmed|15720552]
negative effector of [[protein|CcpN]] activity [Pubmed|15720552]
ADP + Nτ-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase] --> AMP + H+ + Nτ-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase] (according to UniProt)
H+ + Nτ-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase] + phosphate --> diphosphate + Nτ-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase] (according to UniProt)
The protein
Protein family
pyruvate, phosphate dikinase regulatory protein family (according to Swiss-Prot) pyruvate, phosphate dikinase regulatory protein family
pyruvate, phosphate/water dikinase regulatory protein family (single member, according to UniProt)
Biological materials
Mutant
MGNA-C492 (yqfL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2490 NBRP B. subtilis, Japan]
MGNA-C492 (yqfL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2490 NBRP B. subtilis, Japan]
BKE25240 (Δ[[gene|yqfL]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATCCCCCCGTTAGT, downstream forward: _UP4_TAACTCAGGACGCTCTATCC
BKK25240 (Δ[[gene|yqfL]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATCCCCCCGTTAGT, downstream forward: _UP4_TAACTCAGGACGCTCTATCC
Labs working on this gene/protein
[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
References
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|5D0N] (from maize, 39% identity) [pubmed|26620526]
labs
[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France