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Tue Dec 13 2016 11:16:35 GMT+0100 (CET)2025-05-30 02:53:45

description

negative regulator of FtsZ ring formation

negative regulator of [[protein|FtsZ ]]ring formation

locus

BSU29610

BSU_29610

function

control of FtsZ ring formation

control of [[protein|FtsZ ]]ring formation

product

FtsZ-interacting protein

[[protein|FtsZ]]-interacting protein, member of the [SW|divisome]

ec

outlinks

bsu

BSU29610

BSU_29610

aminos

MEFVIGLLIVLLALFAAGYFFRKKIYAEIDRLESWKIEILNRSIVEEMSKIKHLKMTGQTEEFFEKWREEWDEIVTAHMPKVEELLYDAEENADKYRFKKANQVLVHIDDLLTAAESSIEKILREISDLVTSEEKSREEIEQVRERYSKSRKNLLAYSHLYGELYDSLEKDLDEIWSGIKQFEEETEGGNYITARKVLLEQDRNLERLQSYIDDVPKLLADCKQTVPGQIAKLKDGYGEMKEKGYKLEHIQLDKELENLSNQLKRAEHVLMTELDIDEASAILQLIDENIQSVYQQLEGEVEAGQSVLSKMPELIIAYDKLKEEKEHTKAETELVKESYRLTAGELGKQQAFEKRLDEIGKLLSSVKDKLDAEHVAYSLLVEEVASIEKQIEEVKKEHAEYRENLQALRKEELQARETLSNLKKTISETARLLKTSNIPGIPSHIQEMLENAHHHIQETVNQLNELPLNMEEAGAHLKQAEDIVNRASRESEELVEQVILIEKIIQFGNRFRSQNHILSEQLKEAERRFYAFDYDDSYEIAAAAVEKAAPGAVEKIKADISA

[SW|Categories] containing this gene/protein

[SW|cell division], [SW|membrane proteins]

Gene

Coordinates on the chromosome (coding sequence)

3,029,729 -> 3,031,417

Gene

Phenotypes of a mutant

altered localization of [[protein|FtsZ]] and [[ponA|PBP1]] [Pubmed|25403286]

growth defects, elongated cells [Pubmed|25403286]

the ''[[protein|ezrA]]'' mutation is synthetically lethal with a ''[[protein|sepF]]'' mutation [Pubmed|24218584]

a ''[[protein|yvcL]] [[protein|ezrA]]'' double mutant grows poorly, and the cells are filamentous [Pubmed|24097947]

a ''[[protein|zapA]] [[protein|ezrA]]'' double mutant forms filamentous cells [Pubmed|16420366]

depletion of ''[[protein|pdhA]]'' and deletion of ''[[protein|ezrA]]'' have a strong synthetic defect in [SW|cell division] [Pubmed|24825009]

altered localization of [[protein|FtsZ]] and [[gene|ponA|PBP1]]]] [Pubmed|25403286]

growth defects, elongated cells [Pubmed|25403286]

the ''[[gene|ezrA]]'' mutation is synthetically lethal with a ''[[gene|sepF]]'' mutation [Pubmed|24218584]

a ''[[gene|whiA]] [[gene|ezrA]]'' double mutant grows poorly, and the cells are filamentous [Pubmed|24097947]

a ''[[gene|zapA]] [[gene|ezrA]]'' double mutant forms filamentous cells [Pubmed|16420366]

depletion of ''[[gene|pdhA]]'' and deletion of ''[[gene|ezrA]]'' have a strong synthetic defect in [SW|cell division] [Pubmed|24825009]

The protein

Catalyzed reaction/ biological activity

inhibits [[protein|FtsZ]] polymerization [Pubmed|25403286]

recruits [[ponA|PBP1]] to the division septum [Pubmed|25403286]

activates [[protein|PrkC]] [Pubmed|25845974]

inhibits [[protein|FtsZ]] polymerization [Pubmed|25403286]

recruits [[gene|ponA|PBP1]]]] to the division septum [Pubmed|25403286]

activates [[protein|PrkC]] [Pubmed|25845974]

The protein

Protein family

ezrA family (according to Swiss-Prot)

EzrA family (single member, according to UniProt)

The protein

[SW|Localization]

membrane associated [Pubmed|18763711]

1 transmembrane domain at the N-terminus [Pubmed|25403286]

cell membrane (integral) [Pubmed|10449747]

1 transmembrane domain at the N-terminus [Pubmed|25403286]

The protein

[SW|Interactions]

[[protein|EzrA]]-[[protein|UgtP]] [Pubmed|17662947]

[[protein|FtsZ]]-[[protein|EzrA]] [Pubmed|25403286,10449747]

[[protein|EzrA]]-[[protein|GpsB]] [Pubmed|18363795]

[[protein|EzrA]]-[[protein|PonA]] [Pubmed|18363795]

Expression and Regulation

Operon

''[[protein|ezrA]]'' [Pubmed|9387221]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|15317798]

Expression and Regulation

Regulation

constitutively expressed [Pubmed|23701187]

Expression and Regulation

Additional information

degradation of [[protein|EzrA]] involves [[protein|FtsH]] [PubMed|24222488]

Biological materials

Mutant

1A805 ( ''ezrA''::''kan''), [Pubmed|15101985], available at [http://bgsc.org BGSC]

1A1279 (''ezrA''::''spec''), [Pubmed|10449747], available at [http://bgsc.org BGSC]

1A1280 (''ezrA-gfp''), [Pubmed|10449747], available at [http://bgsc.org BGSC]

MGNA-A428 (ytwP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/428 NBRP B. subtilis, Japan]

1A805 ( ''ezrA''::''kan''), [Pubmed|15101985], available at [http://bgsc.org BGSC]

1A1279 (''ezrA''::''spec''), [Pubmed|10449747], available at [http://bgsc.org BGSC]

1A1280 (''ezrA-gfp''), [Pubmed|10449747], available at [http://bgsc.org BGSC]

BKE29610 (Δ[[gene|ezrA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGAGCCCCCTTGCTG, downstream forward: _UP4_TAGATAATCACGACCATGAA

BKK29610 (Δ[[gene|ezrA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGAGCCCCCTTGCTG, downstream forward: _UP4_TAGATAATCACGACCATGAA

References

Reviews

19680248, 19884039

19680248, 19884039, 28357277, 31405912

References

Original Publications

10449747, 16420366, 15317782, 16549676, 17662947, 12368265, 18363795, 9387221, 15317798, 19429628, 18763711, 21401734, 24222488, 23701187, 17718511, 24218584, 24097947, 24825009, 25068683, 25403286, 25845974, 25954268

10449747, 16420366, 15317782, 16549676, 17662947, 12368265, 18363795, 9387221, 15317798, 19429628, 18763711, 21401734, 24222488, 23701187, 17718511, 24218584, 24097947, 24825009, 25068683, 25403286, 25845974, 25954268, 31287946

proteinLength

562

geneLength

1689

Gene

Coordinates

3,029,729 → 3,031,417

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]