Fri May 27 2016 15:40:10 GMT+0200 (CEST)2025-05-18 10:35:23
description
two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay
two-component sensor kinase, phosphorylates [[protein|Spo0F]], part of the [SW|phosphorelay]
locus
BSU13990
BSU_13990
ec
outlinks
bsu
BSU13990
BSU_13990
[SW|Categories] containing this gene/protein
[SW|protein modification], [SW|transcription factors and their control], [SW|phosphorelay], [SW|phosphoproteins]
Gene
Coordinates on the chromosome (coding sequence)
1,470,026 -> 1,471,846
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [SW|Spo0F]
mainly active in the older, inner regions of a colony (with [[protein|KinB]]) [Pubmed|21097618]
autophosphorylation, phosphorylation of [[protein|Spo0F]]
mainly active in the older, inner regions of a colony (with [[protein|KinB]]) [Pubmed|21097618]
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
The protein
[SW|Domains]
three tandem [SW|PAS domain]s in the N-terminal region of KinA, the [SW|second PAS] domain is the major N-terminal determinant of KinA dimerization [Pubmed|23504013]
the first [SW|PAS domain] is required for NAD( ) binding [Pubmed|23599347]
C-terminal histidine phosphotransferase domain
three tandem [SW|PAS domain]s in the N-terminal region of KinA, the [SW|second PAS] domain is the major N-terminal determinant of KinA dimerization [Pubmed|23504013]
the first [SW|PAS domain] is required for NAD(+) binding [Pubmed|23599347]
3 [SW|PAS domain]s (aa 3-73, aa 140-214, aa 265-335) (according to UniProt)
2 [SW|PAC domain]s (aa 77-116, aa 218-255) (according to UniProt)
[SW|Histidine Kinase domain] (aa 402-606) (according to UniProt)
The protein
Effectors of protein activity
interaction with [[protein|KipI]] or [[protein|Sda]] inhibits autophosphorylation of [[protein|KinA]] [Pubmed|9334321,15023339]
autophosphorylation is inhibited by [[protein|SivA]] and [[protein|BslA]] [Pubmed|23335417]
activated by interaction with NAD( ) that indicated poor respiratory activity of the cell [Pubmed|23599347]
interaction with [[protein|PxpB]] or [[protein|Sda]] inhibits autophosphorylation of [[protein|KinA]] [Pubmed|9334321,15023339]
autophosphorylation is inhibited by [[protein|SivA]] and [[protein|BslA]] [Pubmed|23335417]
activated by interaction with NAD(+) that indicated poor respiratory activity of the cell [Pubmed|23599347], this has been falsified [pubmed|28449380]
The protein
[SW|Interactions]
forms tetramers [Pubmed|21926229]
[[protein|KinA]]-[[protein|KipI]] [Pubmed|9334321]
[[protein|KinA]]-[[protein|Sda]] [Pubmed|15023339]
[SW|Spo0F]-[[protein|KinA]]
Expression and Regulation
Operon
''[[protein|kinA]]'' [Pubmed|1569009]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigH]] [Pubmed|1569009]
Expression and Regulation
Regulation
expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|9644251]
induced upon addition of decoyinine (positive [SW|stringent response]) [Pubmed|23378509]
Expression and Regulation
Regulatory mechanism
[SW|Spo0A]: transcription activation [Pubmed|9644251]
Expression and Regulation
Additional information
promoter-up mutations or high artificial expression that render ''[SW|kinA]'' independent from [SW|SigH], allow [SW|sporulation] at high salinity [Pubmed|26712348]
References
Original Publications
proteinLength
606
geneLength
1821
Gene
Coordinates
1,470,026 → 1,471,846
The protein
Paralogous protein(s)
[[this]]
The protein
additional information
the protein forms tetramers [pubmed|28449380]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
BKE13990 (Δ[[gene|kinA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGAATCCCTCCTTTGCA, downstream forward: _UP4_TTTCCAAAAAAATAAAAACA
BKK13990 (Δ[[gene|kinA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGAATCCCTCCTTTGCA, downstream forward: _UP4_TTTCCAAAAAAATAAAAACA