Mon Aug 31 2015 13:52:02 GMT+0200 (CEST)2025-05-25 00:06:27
locus
BSU01530
BSU_01530
outlinks
bsu
BSU01530
BSU_01530
syns
[SW|Categories] containing this gene/protein
[SW|cell wall/ other], [SW|sporulation proteins]
The protein
Catalyzed reaction/ biological activity
formation of muramic acid delta-lacton in spore cortex peptidoglycan [Pubmed|14679227]
formation of muramic acid delta-lacton in spore cortex peptidoglycan [Pubmed|14679227]
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)
The protein
Protein family
N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)
[SW|N-acetylmuramoyl-L-alanine amidase 3 family] (according to UniProt)
The protein
[SW|Domains]
contains an amidase_3 domain (like [[protein|CwlC]], [[protein|LytC]], [[protein|YqiI]], [[protein|YrvJ]])
contains an amidase_3 domain (like [[protein|CwlC]], [[protein|LytC]], [[protein|YqiI]], [[protein|YrvJ]])
[SW|MurNAc-LAA domain] (aa 43-226) (according to UniProt)
Expression and Regulation
Operon
''[[protein|ybaK]]-[[protein|cwlD]]'' [Pubmed|7559346]
''[[protein|cwlD]]'' [Pubmed|7559346]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigE]] [Pubmed|15699190]
''[[protein|ybaK]]'': [[protein|SigG]] [Pubmed|7559346]
''[[protein|cwlD]]'': [[protein|SigE]] [Pubmed|7559346]
Expression and Regulation
Regulation
expressed during sporulation ([[protein|SigG]], [[protein|SigE]]) [Pubmed|7559346]
repressed when no DNA damage is present ([[protein|LexA]]) [Pubmed|16267290]
Expression and Regulation
Regulatory mechanism
[[protein|LexA]]: transcription repression [Pubmed|16267290]
proteinLength
237
geneLength
714
Gene
Coordinates
156,612 → 157,325
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|4RN7] (from Clostridium difficile, 30% identity, aa 43 - 234)
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
BKE01530 (Δ[[gene|cwlD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCTTCCCCTCCCGCTT, downstream forward: _UP4_TAATGGAGGGTTTTCTTGTG
BKK01530 (Δ[[gene|cwlD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCTTCCCCTCCCGCTT, downstream forward: _UP4_TAATGGAGGGTTTTCTTGTG