Mon Aug 31 2015 13:52:02 GMT+0200 (CEST)2025-05-25 00:06:27

locus

BSU01530

BSU_01530

outlinks

bsu

BSU01530

BSU_01530

syns

[SW|Categories] containing this gene/protein

[SW|cell wall/ other], [SW|sporulation proteins]

The protein

Catalyzed reaction/ biological activity

formation of muramic acid delta-lacton in spore cortex peptidoglycan [Pubmed|14679227]

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

The protein

Protein family

N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)

[SW|N-acetylmuramoyl-L-alanine amidase 3 family] (according to UniProt)

The protein

[SW|Domains]

contains an amidase_3 domain (like [[protein|CwlC]], [[protein|LytC]], [[protein|YqiI]], [[protein|YrvJ]])

[SW|MurNAc-LAA domain] (aa 43-226) (according to UniProt)

Expression and Regulation

Operon

''[[protein|ybaK]]-[[protein|cwlD]]'' [Pubmed|7559346]

''[[protein|cwlD]]'' [Pubmed|7559346]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigE]] [Pubmed|15699190]

''[[protein|ybaK]]'': [[protein|SigG]] [Pubmed|7559346]

''[[protein|cwlD]]'': [[protein|SigE]] [Pubmed|7559346]

Expression and Regulation

Regulation

expressed during sporulation ([[protein|SigG]], [[protein|SigE]]) [Pubmed|7559346]

repressed when no DNA damage is present ([[protein|LexA]]) [Pubmed|16267290]

Expression and Regulation

Regulatory mechanism

[[protein|LexA]]: transcription repression [Pubmed|16267290]

proteinLength

237

geneLength

714

Gene

Coordinates

156,612 → 157,325

The protein

Paralogous protein(s)

[[this]]

The protein

Structure

[PDB|4RN7] (from Clostridium difficile, 30% identity, aa 43 - 234)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE01530 (Δ[[gene|cwlD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCTTCCCCTCCCGCTT, downstream forward: _UP4_TAATGGAGGGTTTTCTTGTG

BKK01530 (Δ[[gene|cwlD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCTTCCCCTCCCGCTT, downstream forward: _UP4_TAATGGAGGGTTTTCTTGTG