2017-07-18 12:19:292025-05-26 04:12:11
locus
BSU16420
BSU_16420
geneLength
1071
1074
outlinks
bsu
BSU16420
BSU_16420
The protein
Catalyzed reaction/ biological activity
Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol (according to Swiss-Prot)
[protein]-L-glutamate 5-O-methyl ester + H2O --> H+ + L-glutamyl-[protein] + methanol (according to UniProt)
H2O + L-glutaminyl-[protein] --> L-glutamyl-[protein] + NH4+ (according to UniProt)
The protein
Structure
[PDB|1A2O] (41% identity)
[PDB|1A2O] (from Salmonella enterica, 41% identity) [pubmed|9465023]
The protein
[SW|Localization]
cytoplasm (according to Swiss-Prot)
cytoplasm (according to UniProt)
Biological materials
Mutant
DS7306 (marker-less in NCIB3610) [Pubmed|25313396]
DS7306 (marker-less in NCIB3610) [Pubmed|25313396]
BKE16420 (Δ[[gene|cheB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCAACTACAAGCACACGAA, downstream forward: _UP4_AAAAAAGAGAGGGTGTGATT
BKK16420 (Δ[[gene|cheB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCAACTACAAGCACACGAA, downstream forward: _UP4_AAAAAAGAGAGGGTGTGATT
Labs working on this gene/protein
[SW|Daniel Kearns]
References
Original publications
The protein
Protein family
CheB family (single member, according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|Response regulatory domain] (aa 3-120) (according to UniProt)
CheB-type methyleserase domain (aa 161-355) (according to UniProt)
labs
[SW|Daniel Kearns]