2017-07-18 12:19:292025-05-26 04:12:11

locus

BSU16420

BSU_16420

geneLength

1071

1074

outlinks

bsu

BSU16420

BSU_16420

The protein

Catalyzed reaction/ biological activity

Protein L-glutamate O(5)-methyl ester + H₂O = protein L-glutamate + methanol (according to Swiss-Prot)

[protein]-L-glutamate 5-O-methyl ester + H₂O --> H⁺ + L-glutamyl-[protein] + methanol (according to UniProt)

H₂O + L-glutaminyl-[protein] --> L-glutamyl-[protein] + NH₄⁺ (according to UniProt)

The protein

Structure

[PDB|1A2O] (41% identity)

[PDB|1A2O] (from Salmonella enterica, 41% identity) [pubmed|9465023]

The protein

[SW|Localization]

cytoplasm (according to Swiss-Prot)

cytoplasm (according to UniProt)

Biological materials

Mutant

DS7306 (marker-less in NCIB3610) [Pubmed|25313396]

BKE16420 (Δ[[gene|cheB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCAACTACAAGCACACGAA, downstream forward: _UP4_AAAAAAGAGAGGGTGTGATT

BKK16420 (Δ[[gene|cheB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCAACTACAAGCACACGAA, downstream forward: _UP4_AAAAAAGAGAGGGTGTGATT

Labs working on this gene/protein

[SW|Daniel Kearns]

References

Original publications

25313396, 10632876, 12123464, 1905718, 14731274, 17850253, 14651647, 9657996, 8157612, 15175317, 11553614, 21515776, 24386445

25313396, 10632876, 12123464, 1905718, 14731274, 17850253, 14651647, 9657996, 8157612, 15175317, 11553614, 21515776, 24386445, 9465023

The protein

Protein family

CheB family (single member, according to UniProt)

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|Response regulatory domain] (aa 3-120) (according to UniProt)

CheB-type methyleserase domain (aa 161-355) (according to UniProt)

labs

[SW|Daniel Kearns]