Tue Dec 13 2016 11:16:32 GMT+0100 (CET)2025-05-10 19:22:57
locus
BSU13170
BSU_13170
function
deamination of guanine to xanthine,
purine salvage and interconversion
deamination of guanine to xanthine, purine salvage and interconversion
outlinks
bsu
BSU13170
BSU_13170
aminos
MNHETFLKRAVTLACEGVNAGIGGPFGAVIVKDGAIIAEGQNNVTTSNDPTAHAEVTAIRKACKVLGAYQLDDCILYTSCEPCPMCLGAIYWARPKAVFYAAEHTDAAEAGFDDSFIYKEIDKPAEERTIPFYQVTLTEHLSPFQAWRNFANKKEY
[SW|Categories] containing this gene/protein
[SW|biosynthesis/ acquisition of nucleotides], [SW|general stress proteins (controlled by SigB)], [SW|resistance against oxidative and electrophile stress]
Gene
Coordinates on the chromosome (coding sequence)
1,382,677 -> 1,383,147
The protein
Catalyzed reaction/ biological activity
Guanine + H2O = xanthine + NH3 (according to Swiss-Prot)
guanine + H+ + H2O --> NH4+ + xanthine (according to UniProt)
The protein
Protein family
cytidine and deoxycytidylate deaminase family (according to Swiss-Prot)
[SW|Cytidine and deoxycytidylate deaminase family] (according to UniProt)
Expression and Regulation
Operon
''guaD'' [Pubmed|11101664]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|11101664], [[protein|SigB]] [Pubmed|15805528]
Expression and Regulation
Regulation
induced by stress ([[protein|SigB]]) [Pubmed|15805528]
induced in the presence of purine nucleotides (inducer: allantoin) ([[protein|PucR]]) [Pubmed|12029039]
Expression and Regulation
Regulatory mechanism
[[protein|PucR]]: transcription activation [Pubmed|12029039]
proteinLength
156
geneLength
471
Gene
Coordinates
1,382,677 → 1,383,147
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|CMP/dCMP-type deaminase domain] (aa 1-132) (according to UniProt)
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-A752 (yknA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/752 NBRP B. subtilis, Japan]
BKE13170 (Δ[[gene|guaD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCAATCCCTCCTGTCA, downstream forward: _UP4_TAAAAGGATCAGGCATGCGC
BKK13170 (Δ[[gene|guaD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCAATCCCTCCTGTCA, downstream forward: _UP4_TAAAAGGATCAGGCATGCGC