SubtiBank SubtiBank
Version comparison:

2019-05-24 09:45:232019-02-19 20:12:48

The protein

Paralogous protein(s)

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Biological materials

Expression vectors

GP1477 (chromosomal ''accB''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

pGP1027: IPTG inducible expression, purification in ''E. coli'' without any tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s lab

pGP2649: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab

pGP2650: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab

pGP2652: IPTG inducible expression, purification in ''E. coli'' with C-terminal Strep-tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s lab

GP1477 (chromosomal ''accB''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jrg Stlke]'s lab

pGP1027: IPTG inducible expression, purification in ''E. coli'' without any tag, in [SW|pGP574], available in [SW|Jrg Stlke]'s lab

pGP2649: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jrg Stlke]'s lab

pGP2650: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jrg Stlke]'s lab

pGP2652: IPTG inducible expression, purification in ''E. coli'' with C-terminal Strep-tag, in [SW|pGP574], available in [SW|Jrg Stlke]'s lab

Biological materials

GFP fusion

GP1493 (mls, based on [SW|pGP1080]), available in [SW|Jörg Stülke]'s lab

GP1493 (mls, based on [SW|pGP1080]), available in [SW|Jrg Stlke]'s lab

Biological materials

two-hybrid system

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]'s lab

Biological materials

FLAG-tag construct

GP1484 (''mls'', based on [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab

GP1484 (''mls'', based on [SW|pGP1087]), available in [SW|Jrg Stlke]'s lab