2019-05-24 09:45:232019-02-19 20:12:48
The protein
Paralogous protein(s)
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Biological materials
Expression vectors
GP1477 (chromosomal ''accB''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
pGP1027: IPTG inducible expression, purification in ''E. coli'' without any tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s lab
pGP2649: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab
pGP2650: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
pGP2652: IPTG inducible expression, purification in ''E. coli'' with C-terminal Strep-tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s lab
GP1477 (chromosomal ''accB''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jrg Stlke]'s lab
pGP1027: IPTG inducible expression, purification in ''E. coli'' without any tag, in [SW|pGP574], available in [SW|Jrg Stlke]'s lab
pGP2649: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jrg Stlke]'s lab
pGP2650: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jrg Stlke]'s lab
pGP2652: IPTG inducible expression, purification in ''E. coli'' with C-terminal Strep-tag, in [SW|pGP574], available in [SW|Jrg Stlke]'s lab
Biological materials
GFP fusion
GP1493 (mls, based on [SW|pGP1080]), available in [SW|Jörg Stülke]'s lab
GP1493 (mls, based on [SW|pGP1080]), available in [SW|Jrg Stlke]'s lab
Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]'s lab
Biological materials
FLAG-tag construct
GP1484 (''mls'', based on [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab
GP1484 (''mls'', based on [SW|pGP1087]), available in [SW|Jrg Stlke]'s lab