SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:31 GMT+0100 (CET)2025-05-30 09:52:21

locus

BSU06360

BSU_06360

outlinks

bsu

BSU06360

BSU_06360

aminos

MTKLVNEMILVLDFGSQYNQLITRRIREFGVYSELHPHTLTAEEIKKMNPKGIILSGGPNSVYDENSFRCDEKIFELDIPVLGICYGMQLMTHYLGGKVEAASQREYGKANIRIEGTPDLFRDLPNEQVVWMSHGDLVVEVPEGFTVDATSHHCPNSAMSKADKKWYGVQFHPEVRHSEYGNDLLKNFVFGVCECEGEWSMENFIEIEMQKIRETVGDKQVLCALSGGVDSSVVAVLIHKAIGDQLTCIFVDHGLLRKGEAEGVMKTFSEGFNMNVIKVDAKDRFLNKLKGVSDPEQKRKIIGNEFIYVFDDEADKLKGIDYLAQGTLYTDIIESGTATAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGTELGIPDEIVWRQPFPGPGLGIRVLGEVTEEKLEIVRESDAILREEIANHGLERDIWQYFTVLPDIRSVGVMGDARTYDYTIGIRAVTSIDGMTSDWARIPWDVLEVISTRIVNEVKHINRVVYDITSKPPATIEWE

[SW|Categories] containing this gene/protein

[SW|biosynthesis/ acquisition of nucleotides], [SW|most abundant proteins]

Gene

Coordinates on the chromosome (coding sequence)

692,740 -> 694,281

The protein

Catalyzed reaction/ biological activity

ATP xanthosine 5'-phosphate L-glutamine H2O = AMP diphosphate GMP L-glutamate (according to Swiss-Prot)

ATP + H2O + L-glutamine + XMP --> AMP + diphosphate + GMP + 2 H+ + L-glutamate (according to UniProt)

Expression and Regulation

Operon

''guaA'' [Pubmed|1312531]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|1312531]

Expression and Regulation

Additional information

term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of ''guaA'' [Pubmed|27120414]

belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

References

Original publications

1722815, 1312531, 409404, 15378759, 24682323, 27120414

1722815, 1312531, 409404, 15378759, 24682323, 27120414, 30974118

proteinLength

513

geneLength

1542

Gene

Coordinates

692,740 694,281

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|Glutamine amidotransferase type-1 domain] (aa 8-198) (according to UniProt)

GMPS ATP-PPase domain (aa 199-388) (according to UniProt)

The protein

additional information

belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-A918 (yebB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/918 NBRP B. subtilis, Japan]

BKE06360 ([[gene|guaA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTTGTCACCTAATCTC, downstream forward: _UP4_TAAGAATCAATTAATGGAAA

BKK06360 ([[gene|guaA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTTGTCACCTAATCTC, downstream forward: _UP4_TAAGAATCAATTAATGGAAA