Sun Aug 21 2016 20:49:38 GMT+0200 (CEST)2025-08-21 07:59:05
locus
BSU00090
BSU_00090
essential
yes
no
outlinks
bsu
BSU00090
BSU_00090
[SW|Categories] containing this gene/protein
[SW|biosynthesis/ acquisition of nucleotides], [SW|essential genes], [SW|phosphoproteins], [SW|most abundant proteins]
Gene
Coordinates on the chromosome (coding sequence)
15,915 -> 17,381
Gene
Phenotypes of a mutant
essential [Pubmed|12682299]
reduced expression of ''[[protein|guaB]]'' suppresses the requirement of a ''[[protein|relA]] [[protein|sasA]] [[protein|sasB]]'' triple mutant for branched chain amino acids, methionine and threonine [Pubmed|24163341]
essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]
reduced expression of ''[[gene|guaA]]'' suppresses the requirement of a ''[[gene|relA]] [[gene|sasA]] [[gene|sasB]]'' triple mutant for branched chain amino acids, methionine and threonine [Pubmed|24163341]
The protein
Catalyzed reaction/ biological activity
Inosine 5'-phosphate NAD H2O = xanthosine 5'-phosphate NADH (according to Swiss-Prot)
Inosine 5'-phosphate + NAD+ + H2O --> xanthosine 5'-phosphate + NADH (according to UniProt)
The protein
Protein family
IMPDH/GMPR family (according to Swiss-Prot)
IMPDH/GMPR family (together with [[protein|GuaC]]) (according to UniProt)
The protein
[SW|Domains]
2 [SW|CBS domain]s (95-153, 157-214)
2 [SW|CBS domain]s (aa 95-153, aa 157-214) (according to UniProt)
The protein
Structure
[PDB|1VRD] (from ''Thermotoga maritima msb8'', 60% identity, 80% similarity)
[PDB|3TSB] (from ''B. anthracis'', 80% identity) [pubmed|22788966]
Expression and Regulation
Operon
''guaB'' [Pubmed|22383849]
Expression and Regulation
Regulation
activated during growth in the presence of branched chain amino acids ([[protein|CodY]]) [Pubmed|12618455]
Expression and Regulation
Regulatory mechanism
[[protein|CodY]]: transcription activation [Pubmed|12618455]
Expression and Regulation
Additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
inhibition of enzymatic activity by (p)ppGpp during the 'stringent response' [PubMed|6111556]
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
the mRNA is cleaved by [[rnc|RNase III]] [PubMed|26883633]
the mRNA is very stable (> 15 min) [pubmed|12884008]
the mRNA is cleaved by [[protein|Rnc|RNase III]] [PubMed|26883633]
Biological materials
Expression vector
purification from ''B. subtilis'' with an N-terminal Strep-tag, for [SW|SPINE], (in [SW|pGP380]): pGP901, available in [SW|Jörg Stülke]'s lab
pGP2937 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
References
proteinLength
488
geneLength
1467
Gene
Coordinates
15,915 17,381
The protein
Paralogous protein(s)
[[this]]
The protein
additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
BKE00090 ([[gene|guaA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAGTAAATCCCCCTCTT, downstream forward: _UP4_TAATAAATTGTTACAAATTA
BKK00090 ([[gene|guaA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAGTAAATCCCCCTCTT, downstream forward: _UP4_TAATAAATTGTTACAAATTA
Biological materials
Expression vectors
purification from ''B. subtilis'' with an N-terminal Strep-tag, for [SW|SPINE], (in [SW|pGP380]): pGP901, available in [SW|Jörg Stülke]'s lab
pGP2937 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab