SubtiBank SubtiBank
Version comparison:

Sun Aug 21 2016 20:49:38 GMT+0200 (CEST)2025-08-21 07:59:05

locus

BSU00090

BSU_00090

essential

yes

no

outlinks

bsu

BSU00090

BSU_00090

[SW|Categories] containing this gene/protein

[SW|biosynthesis/ acquisition of nucleotides], [SW|essential genes], [SW|phosphoproteins], [SW|most abundant proteins]

Gene

Coordinates on the chromosome (coding sequence)

15,915 -> 17,381

Gene

Phenotypes of a mutant

essential [Pubmed|12682299]

reduced expression of ''[[protein|guaB]]'' suppresses the requirement of a ''[[protein|relA]] [[protein|sasA]] [[protein|sasB]]'' triple mutant for branched chain amino acids, methionine and threonine [Pubmed|24163341]

essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]

reduced expression of ''[[gene|guaA]]'' suppresses the requirement of a ''[[gene|relA]] [[gene|sasA]] [[gene|sasB]]'' triple mutant for branched chain amino acids, methionine and threonine [Pubmed|24163341]

The protein

Catalyzed reaction/ biological activity

Inosine 5'-phosphate NAD H2O = xanthosine 5'-phosphate NADH (according to Swiss-Prot)

Inosine 5'-phosphate + NAD+ + H2O --> xanthosine 5'-phosphate + NADH (according to UniProt)

The protein

Protein family

IMPDH/GMPR family (according to Swiss-Prot)

IMPDH/GMPR family (together with [[protein|GuaC]]) (according to UniProt)

The protein

[SW|Domains]

2 [SW|CBS domain]s (95-153, 157-214)

2 [SW|CBS domain]s (aa 95-153, aa 157-214) (according to UniProt)

The protein

Structure

[PDB|1VRD] (from ''Thermotoga maritima msb8'', 60% identity, 80% similarity)

[PDB|3TSB] (from ''B. anthracis'', 80% identity) [pubmed|22788966]

Expression and Regulation

Operon

''guaB'' [Pubmed|22383849]

Expression and Regulation

Regulation

activated during growth in the presence of branched chain amino acids ([[protein|CodY]]) [Pubmed|12618455]

Expression and Regulation

Regulatory mechanism

[[protein|CodY]]: transcription activation [Pubmed|12618455]

Expression and Regulation

Additional information

the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

inhibition of enzymatic activity by (p)ppGpp during the 'stringent response' [PubMed|6111556]

belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

the mRNA is cleaved by [[rnc|RNase III]] [PubMed|26883633]

the mRNA is very stable (> 15 min) [pubmed|12884008]

the mRNA is cleaved by [[protein|Rnc|RNase III]] [PubMed|26883633]

Biological materials

Expression vector

purification from ''B. subtilis'' with an N-terminal Strep-tag, for [SW|SPINE], (in [SW|pGP380]): pGP901, available in [SW|Jörg Stülke]'s lab

pGP2937 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

References

22938038, 17611193, 12884008, 1722815, 22981860, 12618455, 17726680, 17726680, 6111556, 24163341, 15378759, 24682323, 25572472, 26883633

22938038, 17611193, 12884008, 1722815, 22981860, 12618455, 17726680, 17726680, 6111556, 24163341, 15378759, 24682323, 25572472, 26883633, 28189581

proteinLength

488

geneLength

1467

Gene

Coordinates

15,915 17,381

The protein

Paralogous protein(s)

[[this]]

The protein

additional information

belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE00090 ([[gene|guaA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAGTAAATCCCCCTCTT, downstream forward: _UP4_TAATAAATTGTTACAAATTA

BKK00090 ([[gene|guaA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAGTAAATCCCCCTCTT, downstream forward: _UP4_TAATAAATTGTTACAAATTA

Biological materials

Expression vectors

purification from ''B. subtilis'' with an N-terminal Strep-tag, for [SW|SPINE], (in [SW|pGP380]): pGP901, available in [SW|Jörg Stülke]'s lab

pGP2937 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab