SubtiBank SubtiBank
Version comparison:

2017-09-06 11:50:282025-05-10 18:50:35

locus

BSU34450

BSU_34450

geneLength

1419

1422

outlinks

bsu

BSU34450

BSU_34450

Gene

Coordinates

3,536,012 → 3,537,433

3,536,012 3,537,433

The protein

Catalyzed reaction/ biological activity

Sucrose (6)-beta-D-fructofuranosyl-(2→)(n) alpha-D-glucopyranoside = glucose (6)-beta-D-fructofuranosyl-(2→)(n 1) alpha-D-glucopyranoside (according to Swiss-Prot)

[6)-β-D-fructofuranosyl-(2→](n) α-D-glucopyranoside + sucrose --> [6)-β-D-fructofuranosyl-(2→](n+1) α-D-glucopyranoside + D-glucose (according to UniProt)

The protein

Protein family

glycosyl hydrolase 68 family (according to Swiss-Prot)

glycosyl hydrolase 68 family (single member, according to UniProt)

Biological materials

lacZ fusion

pGP564 (in [[protein|pAC7]]), a series of RAT mutations in [[protein|pAC7]], all available in [SW|Stülke] lab

pGP564 (in [SW|pAC7]), a series of RAT mutations in [SW|pAC7], all available in [SW|Jörg Stülke]'s lab

References

Original publications

6402497, 2428811, 18197985, 18957862, 2105292, 11739774, 3039303, 22247149, 26256357, 26600431, 26646447

6402497, 2428811, 18197985, 18957862, 2105292, 11739774, 3039303, 22247149, 26256357, 26600431, 26646447, 28871528, 29131629, 28433290, 29794222, 30301900, 30703417, 32553967

The protein

Paralogous protein(s)

[[this]]

Biological materials

Mutant

BKE34450 ([[gene|sacB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTTCATGTCTCCTTTT, downstream forward: _UP4_TAAAAACGCAAAAGAAAATG

BKK34450 ([[gene|sacB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTTCATGTCTCCTTTT, downstream forward: _UP4_TAAAAACGCAAAAGAAAATG