Mon Aug 31 2015 13:53:46 GMT+0200 (CEST)2025-05-25 19:20:18
locus
BSU12460
BSU_12460
outlinks
bsu
BSU12460
BSU_12460
syns
yjpB
[SW|Categories] containing this gene/protein
[SW|cell wall degradation/ turnover], [SW|phage-related functions], [SW|PBSX prophage]
The protein
Catalyzed reaction/ biological activity
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)
The protein
Paralogous protein(s)
[[protein|CwlA]], [[protein|CwlH]], [[protein|XlyA]]
[[this]]
The protein
[SW|Domains]
contains a N-acetylglucosamine-polymer-binding [SW|LysM domain] [Pubmed|18430080]
contains an amidase_2 domain (like [[protein|BlyA]], [[protein|CwlA]], [[protein|CwlH]], [[protein|XlyB]])
contains a N-acetylglucosamine-polymer-binding [SW|LysM domain] [Pubmed|18430080]
contains an amidase_2 domain (like [[protein|BlyA]], [[protein|CwlA]], [[protein|CwlH]], [[protein|XlyB]])
[SW|N-acetylmuramoyl-L-alanine amidase domain] (aa 40-142) (according to UniProt)
[SW|LysM domain] (aa 177-221) (according to UniProt)
The protein
[SW|Localization]
secreted (according to Swiss-Prot)
secreted (according to UniProt)
proteinLength
317
geneLength
954
Gene
Coordinates
1,317,535 1,318,488
The protein
Protein family
[SW|N-acetylmuramoyl-L-alanine amidase 2 family] (according to UniProt)
The protein
Structure
[PDB|2L47] (from Bacillus phage Gamma, corresponds to aa 3 ... 166, 57% identity)
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Expression and Regulation
additional information
the mRNA is very stable (> 15 min) [pubmed|12884008]
Biological materials
Mutant
BKE12460 ([[gene|xlyB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTATCATCTCTCCTTTT, downstream forward: _UP4_TAAATGAAAAGACGGAAGCT
BKK12460 ([[gene|xlyB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTATCATCTCTCCTTTT, downstream forward: _UP4_TAAATGAAAAGACGGAAGCT