SubtiBank SubtiBank
Version comparison:

Mon Aug 31 2015 13:53:46 GMT+0200 (CEST)2025-05-25 19:20:18

locus

BSU12460

BSU_12460

outlinks

bsu

BSU12460

BSU_12460

syns

yjpB

[SW|Categories] containing this gene/protein

[SW|cell wall degradation/ turnover], [SW|phage-related functions], [SW|PBSX prophage]

The protein

Catalyzed reaction/ biological activity

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

The protein

Paralogous protein(s)

[[protein|CwlA]], [[protein|CwlH]], [[protein|XlyA]]

[[this]]

The protein

[SW|Domains]

contains a N-acetylglucosamine-polymer-binding [SW|LysM domain] [Pubmed|18430080]

contains an amidase_2 domain (like [[protein|BlyA]], [[protein|CwlA]], [[protein|CwlH]], [[protein|XlyB]])

contains a N-acetylglucosamine-polymer-binding [SW|LysM domain] [Pubmed|18430080]

contains an amidase_2 domain (like [[protein|BlyA]], [[protein|CwlA]], [[protein|CwlH]], [[protein|XlyB]])

[SW|N-acetylmuramoyl-L-alanine amidase domain] (aa 40-142) (according to UniProt)

[SW|LysM domain] (aa 177-221) (according to UniProt)

The protein

[SW|Localization]

secreted (according to Swiss-Prot)

secreted (according to UniProt)

proteinLength

317

geneLength

954

Gene

Coordinates

1,317,535 1,318,488

The protein

Protein family

[SW|N-acetylmuramoyl-L-alanine amidase 2 family] (according to UniProt)

The protein

Structure

[PDB|2L47] (from Bacillus phage Gamma, corresponds to aa 3 ... 166, 57% identity)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Expression and Regulation

additional information

the mRNA is very stable (> 15 min) [pubmed|12884008]

Biological materials

Mutant

BKE12460 ([[gene|xlyB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTATCATCTCTCCTTTT, downstream forward: _UP4_TAAATGAAAAGACGGAAGCT

BKK12460 ([[gene|xlyB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTATCATCTCTCCTTTT, downstream forward: _UP4_TAAATGAAAAGACGGAAGCT