Mon Aug 31 2015 13:52:20 GMT+0200 (CEST)2025-05-25 03:24:08
locus
BSU03290
BSU_03290
outlinks
bsu
BSU03290
BSU_03290
syns
nasBD, nirD
[SW|Categories] containing this gene/protein
[SW|respiration], [SW|utilization of nitrogen sources other than amino acids]
The protein
Catalyzed reaction/ biological activity
Ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H (according to Swiss-Prot)
2 H2O + 3 NADP+ + NH4+ --> 5 H+ + 3 NADPH + nitrite (according to UniProt)
2 H2O + 3 NAD+ + NH4+ --> 5 H+ + 3 NADH + nitrite (according to UniProt)
The protein
[SW|Cofactors]
NAD [Pubmed|11289299]
NAD [Pubmed|11289299]
FeS cluster [pubmed|29292548]
The protein
[SW|Interactions]
[[protein|NasD]]-[[protein|NasE]] [Pubmed|11289299]
Expression and Regulation
Operon
''[[protein|nasB]]-[[protein|nasC]]-[[protein|nasD]]-[[protein|nasE]]-[[protein|nasF]]'' [Pubmed|7836289]
''[[protein|nasD]]-[[protein|nasE]]-[[protein|nasF]]'' [Pubmed|9765565]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|7836289]
Expression and Regulation
Regulation
expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[protein|TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114,9765565 PubMed]
''[[protein|nasD]]'': expressed under anaerobic conditions ([[protein|ResD]]) [Pubmed|10972836]
induced by nitric oxide under anaerobic conditions ([[protein|NsrR]]) [Pubmed|16885456]
induced by iron starvation ([[protein|Fur]]) [Pubmed|12354229]
Expression and Regulation
Regulatory mechanism
[[protein|TnrA]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114,9765565 PubMed]
[[protein|ResD]]: transcription activation [Pubmed|10972836]
[[protein|NsrR]]: transcription repression [Pubmed|16885456]
[[protein|Fur]]: transcription repression [Pubmed|12354229]
proteinLength
106
geneLength
321
Gene
Coordinates
355,412 355,732
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
Rieske domain (aa 8-104) (according to UniProt)
The protein
Structure
[PDB|5BOK] (from Diaphorobacter sp., 30% identity)
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
BKE03290 ([[gene|nasE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCATTCCTCCTCAAT, downstream forward: _UP4_TGATCAAAAGACCGGATCAC
BKK03290 ([[gene|nasE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCATTCCTCCTCAAT, downstream forward: _UP4_TGATCAAAAGACCGGATCAC