Tue Dec 13 2016 11:16:35 GMT+0100 (CET)2025-05-13 03:53:11
locus
BSU29850
BSU_29850
ec
outlinks
bsu
BSU29850
BSU_29850
aminos
MEFIVYLAGEIHSNWREEIKEKTKSLKLPITFVGPMENHDRSDNIGEEIMGVQPNAVLKDDKASDINNFRTAVLMNKADFVIALFGEKYKQWNTAMDASYAIAKGKPLIIIRPESLHHPLKELSNKANITVETVNQAIKALSYLFETE
[SW|Categories] containing this gene/protein
[SW|proteins of unknown function]
Gene
Coordinates on the chromosome (coding sequence)
3,054,746 -> 3,055,192
proteinLength
148
geneLength
447
Gene
Coordinates
3,054,746 3,055,192
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-B532 (ytoQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1531 NBRP B. subtilis, Japan]
BKE29850 ([[gene|ytoQ]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATAAAAATCCCTCCTT, downstream forward: _UP4_TAAGAAAAAAGCTTGTCGAT
BKK29850 ([[gene|ytoQ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATAAAAATCCCTCCTT, downstream forward: _UP4_TAAGAAAAAAGCTTGTCGAT
BP1101 ([[gene|ytoQ]]::kan trp+) available at [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs
Biological materials
Expression vectors
IPTG inducible expression of Strep-ytoQ in E. coli: pBP641 (in [SW|pGP172]) and pBP642 (in [SW|pGP574]), available in [SW|Fabian Commichau]'s lab
Constitutive expression of ytoQ in B. subtilis: pBP639 (in [SW|pBQ200]), available in [SW|Fabian Commichau]'s lab, [Pubmed|29027347]
pBP770: expression of Strep-''ytoQ'' by [SW|pGP380]in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke's] and [SW|Fabian Commichau]'s labs
pBP779: expression of ''ytoQ''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs
Biological materials
lacZ fusion
pBP638 (in [SW|pAC7]), available in [SW|Fabian Commichau]'s lab [Pubmed|29027347]
labs
[SW|Fabian Commichau], Göttingen, Germany [http://www.uni-goettingen.de/de/413008.html homepage]
References
Research papers