SubtiBank SubtiBank
Version comparison:

2017-04-12 08:23:242025-05-22 13:00:36

locus

BSU29580

BSU_29580

proteinLength

358

401

geneLength

1074

1206

outlinks

bsu

BSU29580

BSU_29580

Gene

Coordinates

3,025,748 -> 3,026,953

3,025,748 3,026,953

The protein

Catalyzed reaction/ biological activity

receives a sulfuryl group from [[protein|NifZ]] and transfers it to uridine at position 8 of tRNA [Pubmed|22773787]

receives a sulfuryl group from [[protein|NifZ]] and transfers it to uridine at position 8 of tRNA [Pubmed|22773787]

[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uridine in tRNA + ATP + H+ + 2 reduced [2Fe-2S]-[ferredoxin] --> [ThiI sulfur-carrier protein]-L-cysteine + 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)

[sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] --> [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H+ + L-cysteinyl-[cysteine desulfurase] (according to UniProt)

The protein

Protein family

thiI family (according to Swiss-Prot)

thiI family (single member, according to UniProt)

The protein

Structure

[PDB|2C5S] (from B. anthracis)

[PDB|4KR6] (ThiI-RNA complex from Thermotoga maritima) [pubmed|24705700]

[PDB|2C5S] (from B. anthracis) [pubmed|16343540]

References

14567704, 22773787

14567704, 22773787, 16343540, 24705700

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

THUMP domain (aa 60-165) (according to UniProt)

Biological materials

Mutant

BKE29580 ([[gene|thiI]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATATATGATCGTAATTCA, downstream forward: _UP4_TAAATCAATTTTCAGCTCCT

BKK29580 ([[gene|thiI]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATATATGATCGTAATTCA, downstream forward: _UP4_TAAATCAATTTTCAGCTCCT