2017-04-12 08:23:242025-05-22 13:00:36
locus
BSU29580
BSU_29580
proteinLength
358
401
geneLength
1074
1206
outlinks
bsu
BSU29580
BSU_29580
Gene
Coordinates
3,025,748 -> 3,026,953
3,025,748 3,026,953
The protein
Catalyzed reaction/ biological activity
receives a sulfuryl group from [[protein|NifZ]] and transfers it to uridine at position 8 of tRNA [Pubmed|22773787]
receives a sulfuryl group from [[protein|NifZ]] and transfers it to uridine at position 8 of tRNA [Pubmed|22773787]
[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uridine in tRNA + ATP + H+ + 2 reduced [2Fe-2S]-[ferredoxin] --> [ThiI sulfur-carrier protein]-L-cysteine + 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)
[sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] --> [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H+ + L-cysteinyl-[cysteine desulfurase] (according to UniProt)
The protein
Protein family
thiI family (according to Swiss-Prot)
thiI family (single member, according to UniProt)
The protein
Structure
[PDB|2C5S] (from B. anthracis)
[PDB|4KR6] (ThiI-RNA complex from Thermotoga maritima) [pubmed|24705700]
[PDB|2C5S] (from B. anthracis) [pubmed|16343540]
References
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
THUMP domain (aa 60-165) (according to UniProt)
Biological materials
Mutant
BKE29580 ([[gene|thiI]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATATATGATCGTAATTCA, downstream forward: _UP4_TAAATCAATTTTCAGCTCCT
BKK29580 ([[gene|thiI]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATATATGATCGTAATTCA, downstream forward: _UP4_TAAATCAATTTTCAGCTCCT