Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)2025-05-30 07:21:21
description
undecaprenyl pyrophosphate phosphatase, produces the carrier lipid for cell wall synthesis, bacitracin resistance
undecaprenyl pyrophosphate phosphatase, produces the carrier lipid for cell wall synthesis, secondary bacitracin resistance determinant
locus
BSU36530
BSU_36530
outlinks
bsu
BSU36530
BSU_36530
aminos
MNYEIFKAIHGLSHHNSVLD SIMVFITEYA IVAYALILLA IWLFGNTQSR KHVLYAGITG IAGLVINYLI TLVYFEPRPF VAHTVHTLIP HAADASFPSD HTTGALAISI AMLFRNRKIG WPLVIFGLLT GFSRIWVGHH YPVDVLGSLV VAIIIGFLFF RFSDLLRPFV DLVVRIYEAI INKLTKKPTD QNF
[SW|Categories] containing this gene/protein
[SW|cell wall synthesis], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)], [SW|heat shock proteins], [SW|resistance against toxins/ antibiotics], [SW|membrane proteins]
Gene
Coordinates on the chromosome (coding sequence)
3,758,547 -> 3,759,128
Gene
Phenotypes of a mutant
increased susceptibility to rare earth elements [Pubmed|22904278]
a ''[SW|bcrC]'' ''[SW|uppP]'' double mutant is not viable (unless ''[SW|yodM]'' is artificially expressed) [Pubmed|27528508]
5-fold increased sensitivity to bacitracin [Pubmed|26815905]
reduced growth rate [Pubmed|26815905]
increased susceptibility to rare earth elements [Pubmed|22904278]
a ''[[gene|bcrC]]'' ''[[gene|uppP]]'' double mutant is not viable (unless ''[[gene|pgpB]]'' is artificially expressed) [Pubmed|27528508]
The protein
Catalyzed reaction/ biological activity
undecaprenyl pyrophosphate --> undecaprenyl phosphate
undecaprenyl pyrophosphate - undecaprenyl phosphate
di-trans,octa-cis-undecaprenyl diphosphate + H2O --> di-trans,octa-cis-undecaprenyl phosphate + H+ + phosphate (according to UniProt)
The protein
Protein family
bcrC/ybjG/ywoA family (according to Swiss-Prot)
BcrC/YbjG family (single member, according to UniProt)
The protein
Effectors of protein activity
inhibited by heptaprenyl pyrophosphate which accumulates in a ''[[protein|ytpB]]'' mutant [Pubmed|24806199]
inhibited by heptaprenyl pyrophosphate which accumulates in a ''[[gene|ytpB]]'' mutant [Pubmed|24806199]
The protein
Additional information
there is a second undecaprenyl pyrophosphate phosphatase in ''B. subtilis'', [[protein|YubB]]. However, [[protein|YubB]] seems to play only a minor role. [Pubmed|22904278]
there is a second undecaprenyl pyrophosphate phosphatase in ''B. subtilis'', [[protein|UppP]]. However, [[protein|UppP]] seems to play only a minor role. [Pubmed|22904278]
Expression and Regulation
Operon
''bcrC
Expression and Regulation
[SW|Sigma factor]
[[protein|SigM]] [Pubmed|17434969], [[protein|SigI]] [Pubmed|18156261], [[protein|SigW]] [Pubmed|11866510], [[protein|SigX]] [Pubmed|12399481]
Expression and Regulation
Regulation
expression activated by glucose (3.3 fold) [Pubmed|12850135]
induced by heat shock ([[protein|SigI]]) [Pubmed|18156261]
induced by bacitracin
induced by oxidative stress
induced in the stationary phase due to the internal production of toxic peptides ([SW|SdpC], [SW|SkfA]) [Pubmed|26364265]
expression is reduced in a [[protein|SigV]] mutant [Pubmed|21926231]
Biological materials
Mutant
BKE36530 ''bcrC''::''erm trpC2'' is available from the Bacillus Genetic Stock Center
MGNA-A214 (ywoA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/214 NBRP B. subtilis, Japan]
BKE36530 ([[gene|bcrC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATAATCACCTTTTACAT, downstream forward: _UP4_TAAGAAAGACAAAAGCCGGC
BKK36530 ([[gene|bcrC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATAATCACCTTTTACAT, downstream forward: _UP4_TAAGAAAGACAAAAGCCGGC
References
Original publications
proteinLength
193
geneLength
582
Gene
Coordinates
3,758,547 3,759,128
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]