Tue Dec 13 2016 11:16:34 GMT+0100 (CET)2025-05-11 15:38:08
locus
BSU24080
BSU_24080
product
valine dehydrogenase, isoleucine dehydrogenase,
L-leucine dehydrogenase
valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase
outlinks
bsu
BSU24080
BSU_24080
aminos
MELFKYMEKYDYEQLVFCQDEQSGLKAIIAIHDTTLGPALGGTRMWTYENEEAAIEDALRLARGMTYKNAAAGLNLGGGKTVIIGDPRKDKNEEMFRAFGRYIQGLNGRYITAEDVGTTVEDMDIIHDETDYVTGISPAFGSSGNPSPVTAYGVYRGMKAAAKAAFGTDSLEGKTIAVQGVGNVAYNLCRHLHEEGANLIVTDINKQSVQRAVEDFGARAVDPDDIYSQDCDIYAPCALGATINDDTIKQLKAKVIAGAANNQLKETRHGDQIHEMGIVYAPDYVINAGGVINVADELYGYNAERALKKVEGIYGNIERVLEISQRDGIPAYLAADRLAEERIERMRRSRSQFLQNGHSVLSRR
[SW|Categories] containing this gene/protein
[SW|utilization of amino acids]
Gene
Coordinates on the chromosome (coding sequence)
2,502,659 -> 2,503,753
The protein
Catalyzed reaction/ biological activity
L-leucine + H2O + NAD+ = 4-methyl-2-oxopentanoate + NH3 + NADH (according to Swiss-Prot)
L-leucine + H2O + NAD+ --> 4-methyl-2-oxopentanoate + NH4+ + NADH + H+ (according to UniProt)
The protein
Protein family
Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)
Glu/Leu/Phe/Val dehydrogenases family (with [[protein|GudB]] and [[protein|RocG]], according to UniProt)
Expression and Regulation
Operon
''[[protein|ptb]]-[[protein|bcd]]-[[protein|buk]]-[[protein|lpdV]]-[SW|bkdAA]-[SW|bkdAB]-[[protein|bkdB]]'' [Pubmed|12823818]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigL]] [Pubmed|10094682]
Expression and Regulation
Regulation
induced in the presence of isoleucine or valine ([[protein|BkdR]]) [Pubmed|10094682]
Expression and Regulation
Regulatory mechanism
[[protein|CodY]]: indirect regulation [Pubmed|24843172]
[[protein|BkdR]]: transcription activation [Pubmed|10094682]
Biological materials
Mutant
a ''bcd'' mutant and a ''[[protein|bcd]] [[protein|ybgE]] [[protein|ywaA]]'' triple mutant are available in [SW|Linc Sonenshein]'s lab
MGNA-C427 (yqiT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2425 NBRP B. subtilis, Japan]
a ''bcd'' mutant and a ''[[gene|bcd]] [[gene|ybgE]] [[gene|ywaA]]'' triple mutant are available in [SW|Linc Sonenshein]'s lab
BKE24080 ([[gene|bcd]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAAAAGTTCCATGTTCGTTC, downstream forward: _UP4_CGTTAAGAAATTGATCTGGA
BKK24080 ([[gene|bcd]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAAAAGTTCCATGTTCGTTC, downstream forward: _UP4_CGTTAAGAAATTGATCTGGA
References
Original publications
proteinLength
364
geneLength
1095
Gene
Coordinates
2,502,659 2,503,753
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]