SubtiBank SubtiBank
Version comparison:

Mon Jun 06 2016 13:10:44 GMT+0200 (CEST)2025-05-11 23:20:07

description

transcription repressor of threonine biosynthetic gene

transcription repressor of threonine biosynthetic genes

locus

BSU27910

BSU_27910

product

transcription repressor of threonine biosynthetic gene

transcription repressor of threonine biosynthetic genes

ec

outlinks

bsu

BSU27910

BSU_27910

[SW|Categories] containing this gene/protein

[SW|transcription factors and their control], [SW|biosynthesis/ acquisition of amino acids]

Gene

Coordinates on the chromosome (coding sequence)

2,852,157 -> 2,852,600

The protein

Catalyzed reaction/ biological activity

control of the threonine biosynthetic genes (''[[protein|hom]]-[[protein|thrC]]-[[protein|thrB]]'' and ''[SW|thrD]'') [Pubmed|27260660]

control of the threonine biosynthetic genes (''[[gene|hom]]-[[gene|thrC]]-[[gene|thrB]]'' and ''[[gene|thrD]]'') [Pubmed|27260660]

The protein

Protein family

UPF0735 family (according to Swiss-Prot)

UPF0735 family (single member, according to UniProt)

The protein

[SW|Domains]

putative DNA binding signature (aa 19-51)

ACT (aspartate kinase-chorismate mutase-tyrA) domain (aa 70-145)

putative DNA binding signature (aa 19-51)

[SW|ACT domain] (aa 70-145) (according to UniProt)

Expression and Regulation

Operon

''[SW|spo0B]-[[protein|obg]]-[[protein|thrR]]-[[protein|pheA]]'' [Pubmed|22383849]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|2537815]

Biological materials

Mutant

BKE27910 (''[[protein|thrR]]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs) [Pubmed|27260660]

BKE27910 (''[[gene|thrR]]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs) [Pubmed|27260660]

BKE27910 ([[gene|thrR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGCATCCCCCCTTTAA, downstream forward: _UP4_GGTGCATAAGGGAGAGAAAA

BKK27910 ([[gene|thrR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGCATCCCCCCTTTAA, downstream forward: _UP4_GGTGCATAAGGGAGAGAAAA

Biological materials

Expression vector

pGP323 (N-terminal Strep-tag, in [SW|pGP172]) (available in [SW|Fabian Commichau]'s lab) [Pubmed|27260660]

pBP620 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Fabian Commichau]'s lab) [Pubmed|27260660]

pBP622 (C-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP382]) (available in [SW|Fabian Commichau]'s lab) [Pubmed|27260660]

References

2537815, 25777134, 27260660

2537815, 25777134, 27260660, 32743959

proteinLength

147

geneLength

444

Gene

Coordinates

2,852,157 2,852,600

Gene

Phenotypes of a mutant

resistance to serine [pubmed|32743959]

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Expression vectors

pGP2290: expression of ''thrR'' in B. subtilis (in[SW|pBQ200]), available in [SW|Jörg Stülke]'s lab

pBP323 (N-terminal Strep-tag, in [SW|pGP172]) (available in [SW|Fabian Commichau]'s lab) [Pubmed|27260660]

pBP620 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Fabian Commichau]'s lab) [Pubmed|27260660]

pBP622 (C-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP382]) (available in [SW|Fabian Commichau]'s lab) [Pubmed|27260660]

Biological materials

lacZ fusion

pGP2291 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab

labs

[SW|Fabian Commichau] Göttingen, Germany [http://www.uni-goettingen.de/de/413008.html homepage]