Mon Jun 06 2016 13:10:44 GMT+0200 (CEST)2025-05-11 23:20:07
description
transcription repressor of threonine biosynthetic gene
transcription repressor of threonine biosynthetic genes
locus
BSU27910
BSU_27910
product
transcription repressor of threonine biosynthetic gene
transcription repressor of threonine biosynthetic genes
ec
outlinks
bsu
BSU27910
BSU_27910
[SW|Categories] containing this gene/protein
[SW|transcription factors and their control], [SW|biosynthesis/ acquisition of amino acids]
Gene
Coordinates on the chromosome (coding sequence)
2,852,157 -> 2,852,600
The protein
Catalyzed reaction/ biological activity
control of the threonine biosynthetic genes (''[[protein|hom]]-[[protein|thrC]]-[[protein|thrB]]'' and ''[SW|thrD]'') [Pubmed|27260660]
control of the threonine biosynthetic genes (''[[gene|hom]]-[[gene|thrC]]-[[gene|thrB]]'' and ''[[gene|thrD]]'') [Pubmed|27260660]
The protein
Protein family
UPF0735 family (according to Swiss-Prot)
UPF0735 family (single member, according to UniProt)
The protein
[SW|Domains]
putative DNA binding signature (aa 19-51)
ACT (aspartate kinase-chorismate mutase-tyrA) domain (aa 70-145)
putative DNA binding signature (aa 19-51)
[SW|ACT domain] (aa 70-145) (according to UniProt)
Expression and Regulation
Operon
''[SW|spo0B]-[[protein|obg]]-[[protein|thrR]]-[[protein|pheA]]'' [Pubmed|22383849]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|2537815]
Biological materials
Mutant
BKE27910 (''[[protein|thrR]]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs) [Pubmed|27260660]
BKE27910 (''[[gene|thrR]]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs) [Pubmed|27260660]
BKE27910 ([[gene|thrR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGCATCCCCCCTTTAA, downstream forward: _UP4_GGTGCATAAGGGAGAGAAAA
BKK27910 ([[gene|thrR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGCATCCCCCCTTTAA, downstream forward: _UP4_GGTGCATAAGGGAGAGAAAA
Biological materials
Expression vector
pGP323 (N-terminal Strep-tag, in [SW|pGP172]) (available in [SW|Fabian Commichau]'s lab) [Pubmed|27260660]
pBP620 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Fabian Commichau]'s lab) [Pubmed|27260660]
pBP622 (C-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP382]) (available in [SW|Fabian Commichau]'s lab) [Pubmed|27260660]
References
proteinLength
147
geneLength
444
Gene
Coordinates
2,852,157 2,852,600
Gene
Phenotypes of a mutant
resistance to serine [pubmed|32743959]
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Expression vectors
pGP2290: expression of ''thrR'' in B. subtilis (in[SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
pBP323 (N-terminal Strep-tag, in [SW|pGP172]) (available in [SW|Fabian Commichau]'s lab) [Pubmed|27260660]
pBP620 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Fabian Commichau]'s lab) [Pubmed|27260660]
pBP622 (C-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP382]) (available in [SW|Fabian Commichau]'s lab) [Pubmed|27260660]
Biological materials
lacZ fusion
pGP2291 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab
labs
[SW|Fabian Commichau] Göttingen, Germany [http://www.uni-goettingen.de/de/413008.html homepage]