2018-05-16 09:56:392025-05-24 18:12:39
description
unknown, similar to formate dehydrogenase
putative formate dehydrogenase
locus
BSU27220
BSU_27220
geneLength
2940
2943
outlinks
bsu
BSU27220
BSU_27220
Gene
Coordinates
2,781,209 → 2,784,151
2,781,209 2,784,151
The protein
Catalyzed reaction/ biological activity
Formate + NAD+ = CO2 + NADH (according to Swiss-Prot)
formate + NAD+ --> CO2 + NADH (according to UniProt)
The protein
Additional information
The gene is annotated in KEGG as an ortholog of formate dehydrogenase EC 1.2.1.2. In Swiss-Prot the protein is named “formate dehydrogenase chain A”. In MetaCyc the protein is characterized as “similar to formate dehydrogenase”. No literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]
The gene is annotated in KEGG as an ortholog of formate dehydrogenase EC 1.2.1.2. In Swiss-Prot the protein is named formate dehydrogenase chain A. In MetaCyc the protein is characterized as similar to formate dehydrogenase. No literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]
Biological materials
Mutant
MGNA-A160 (yrhE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/160 NBRP B. subtilis, Japan]
BKE27220 (Δ[[gene|yrhE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCATATTCCCCTCCA, downstream forward: _UP4_GATTAAACGATAGGAGGAAA
BKK27220 (Δ[[gene|yrhE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCATATTCCCCTCCA, downstream forward: _UP4_GATTAAACGATAGGAGGAAA
MGNA-A160 (yrhE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/160 NBRP B. subtilis, Japan]
BKE27220 ([[gene|yrhE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCATATTCCCCTCCA, downstream forward: _UP4_GATTAAACGATAGGAGGAAA
BKK27220 ([[gene|yrhE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCATATTCCCCTCCA, downstream forward: _UP4_GATTAAACGATAGGAGGAAA
References
The protein
Protein family
C-terminal part: [SW|prokaryotic molybdopterin-containing oxidoreductase family] (according to UniProt)
The protein
[SW|Domains]
2Fe-2S ferredoxin-type domain (aa 5-81) (according to UniProt)
4Fe-4S His(Cys)3-ligated-type domain (aa 81-121) (according to UniProt)
2 [SW|4Fe-4S ferredoxin-type domain]s (aa 144-171, aa 187-216) (according to UniProt)
[SW|4Fe-4S Mo/W bis-MGD-type domain] (aa 263-319) (according to UniProt)
The protein
Structure
[PDB|2FUG] (from Thermus thermophilus, 23% identity) [pubmed|16469879]