Tue Dec 13 2016 11:16:35 GMT+0100 (CET)2025-05-14 05:46:52
description
similar to protease
tRNA uridine hydroxylase (in complex with [[protein|YrrO]])
locus
BSU27350
BSU_27350
function
unknown
introduction of 5-methoxyuridine modification in tRNA (U34)
product
unknown
tRNA uridine hydroxylase
ec
names
yrrN
yrrN
trhP1
outlinks
bsu
BSU27350
BSU_27350
aminos
MKKPELLVTPTSTADILPLIQAGATAFLVGEQRYGLRLAGEFSREDVTKAVEIAHKEGAKVYVAVNAIFHNDKVGELGEYLAFLAEAGVDAAVFGDPAVLMAARESAPDLKLHWSTETTGTNYYTCNYWGRKGAARSVLARELNMDSIVEIKENAEVEIEIQVHGMTCMFQSKRSLIGNYFEYQGKVMDIERKKKESGMFLHDKERDNKYPIFEDENGTHIMSPNDVCIIDELEELIDAGIDSFKIDGVLKMPEYLIEVTKMYREAIDLCVENRDEYEAKKEDWIERIESIQPVNRKIDTGFFFKETVY
[SW|Categories] containing this gene/protein
[SW|poorly characterized/ putative enzymes]
Gene
Coordinates on the chromosome (coding sequence)
2,794,147 -> 2,795,076
The protein
Protein family
peptidase U32 family (according to Swiss-Prot)
peptidase U32 family (with [[protein|YrrO]], according to UniProt)
The protein
Paralogous protein(s)
[[protein|YrrO]]
[[this]]
proteinLength
309
geneLength
930
Gene
Coordinates
2,794,147 2,795,076
Gene
Phenotypes of a mutant
50% reduction in mo5U tRNA levels [pubmed|31358606]
The protein
Catalyzed reaction/ biological activity
hydroxylation of U34 in tRNA to give 5-hydroxyuridine (ho5U) (in complex with [[protein|YrrO]]) with prephenate as hydroxyl donor, ho5U is the precursor for 5-methoxyuridine modification (by [[protein|TrmR]]) [pubmed|31358606]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-A008 (yrrN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/8 NBRP B. subtilis, Japan]
BKE27350 ([[gene|yrrN]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACGTTCACCTCTTCTT, downstream forward: _UP4_TATTAATCAAAAGGAGGTTA
BKK27350 ([[gene|yrrN]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACGTTCACCTCTTCTT, downstream forward: _UP4_TATTAATCAAAAGGAGGTTA
References
Research papers