Wed Aug 24 2016 13:19:20 GMT+0200 (CEST)2025-05-13 04:40:42
locus
BSU33040
BSU_33040
outlinks
bsu
BSU33040
BSU_33040
[SW|Categories] containing this gene/protein
[SW|carbon core metabolism], [SW|most abundant proteins]
Gene
Coordinates on the chromosome (coding sequence)
3,389,024 -> 3,390,412
The protein
Catalyzed reaction/ biological activity
(S)-malate = fumarate
(S)-malate --> fumarate + H2O (according to UniProt)
The protein
Protein family
Fumarase subfamily (according to Swiss-Prot)
class-II fumarase/aspartase family (with [[protein|AnsB]], according to UniProt)
The protein
Paralogous protein(s)
[[protein|AnsB]]
[[this]]
The protein
Structure
[PDB|3R6Q] (aspartase from ''Bacillus'' sp. YM55-1, 43% identity, 77% similarity) [Pubmed|21661762]
[PDB|1FUO] (from ''E. coli'', 64% identity) [Pubmed|8909293]
The protein
[SW|Localization]
cytoplasm (according to Swiss-Prot)
forms discrete foci in the cytoplasm [pubmed|29140245]
colocalizes with DNA upon DNA damage (MMS treatment) [pubmed|29140245]
The protein
[SW|Interactions]
[[protein|Mdh]]-[[protein|CitG]] [Pubmed|20933603]
[[protein|CitG]]-[[protein|CitB]] [Pubmed|20933603]
Expression and Regulation
Operon
''[[protein|citG]]'' [Pubmed|3923430]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|3130545,2509422,2509423], [[protein|SigH]] [Pubmed|3130545,2509422]
Expression and Regulation
Regulation
constitutive
Expression and Regulation
Additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
the protein amounts increase upon DNA damage (MMS treatment) [pubmed|29140245]
Biological materials
Mutant
GP718 (spec), available in [SW|Jörg Stülke]'s lab
GP2340 (''[[protein|citG]]''::''kan''), Cre-recombinase is integrated in ''[[protein|sacA]]'',available in [SW|Jörg Stülke]'s lab
GP2341 (''[[protein|citG]]''::''lox72''), Cre-recombinase is integrated in ''[[protein|sacA]]'', available in [SW|Jörg Stülke]'s lab
GP718 (spec), available in [SW|Jörg Stülke]'s lab [pubmed|20933603]
GP2340 Δ''[[gene|citG]]''::''kan'', Cre-recombinase is integrated in ''[[gene|sacA]]'', available in [SW|Jörg Stülke]'s lab
GP2341 Δ''[[gene|citG]]''::''lox72'', Cre-recombinase is integrated in ''[[gene|sacA]]'', available in [SW|Jörg Stülke]'s lab
BKE33040 ([[gene|citG]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATGTATCCCTCCATA, downstream forward: _UP4_TAATAGGAAGAACGGCTGCT
BKK33040 ([[gene|citG]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATGTATCCCTCCATA, downstream forward: _UP4_TAATAGGAAGAACGGCTGCT
Biological materials
Expression vector
pGP1122 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab)
Biological materials
lacZ fusion
pGP387 (in [[protein|pAC7]]), available in [SW|Jörg Stülke]'s lab
pGP387 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab
Biological materials
GFP fusion
GP1433 (spc, based on [SW|pGP1870]), available in the [SW|Stülke] lab
GP1433 (spc, based on [SW|pGP1870]), available in the [SW|Jörg Stülke] lab
References
proteinLength
462
geneLength
1389
Gene
Coordinates
3,389,024 3,390,412
The protein
additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Expression vectors
pGP1122 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab) [pubmed|20933603]