SubtiBank SubtiBank
Version comparison:

2017-06-19 10:25:522024-12-18 21:14:34

locus

BSU33040

BSU_33040

geneLength

1386

1389

outlinks

bsu

BSU33040

BSU_33040

Gene

Coordinates

3,389,024 → 3,390,412

3,389,024 3,390,412

The protein

Catalyzed reaction/ biological activity

(S)-malate = fumarate + H2O (according to Swiss-Prot)

(S)-malate --> fumarate + H2O (according to UniProt)

The protein

Protein family

Fumarase subfamily (according to Swiss-Prot)

class-II fumarase/aspartase family (with [[protein|AnsB]], according to UniProt)

The protein

Paralogous protein(s)

[[protein|AnsB]]

[[this]]

The protein

Structure

[PDB|3R6Q] (aspartase from ''Bacillus'' sp. YM55-1, 43% identity, 77% similarity) [Pubmed|21661762]

[PDB|1FUO] (from ''E. coli'', 64% identity) [Pubmed|8909293]

The protein

[SW|Localization]

cytoplasm (according to Swiss-Prot)

forms discrete foci in the cytoplasm [pubmed|29140245]

colocalizes with DNA upon DNA damage (MMS treatment) [pubmed|29140245]

Biological materials

Mutant

GP718 (spec), available in [SW|Jörg Stülke]'s lab

GP2340 (''[[gene|citG]]''::''kan''), Cre-recombinase is integrated in ''[[gene|sacA]]'', available in [SW|Jörg Stülke]'s lab

GP2341 (''[[gene|citG]]''::''lox72''), Cre-recombinase is integrated in ''[[gene|sacA]]'', available in [SW|Jörg Stülke]'s lab

GP718 (spec), available in [SW|Jörg Stülke]'s lab [pubmed|20933603]

GP2340 Δ''[[gene|citG]]''::''kan'', Cre-recombinase is integrated in ''[[gene|sacA]]'', available in [SW|Jörg Stülke]'s lab

GP2341 Δ''[[gene|citG]]''::''lox72'', Cre-recombinase is integrated in ''[[gene|sacA]]'', available in [SW|Jörg Stülke]'s lab

BKE33040 ([[gene|citG]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATGTATCCCTCCATA, downstream forward: _UP4_TAATAGGAAGAACGGCTGCT

BKK33040 ([[gene|citG]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATGTATCCCTCCATA, downstream forward: _UP4_TAATAGGAAGAACGGCTGCT

Biological materials

Expression vector

pGP1122 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab)

Biological materials

lacZ fusion

pGP387 (in [[protein|pAC7]]), available in [SW|Jörg Stülke]'s lab

pGP387 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab

Biological materials

GFP fusion

GP1433 (spc, based on [SW|pGP1870]), available in the [SW|Stülke] lab

GP1433 (spc, based on [SW|pGP1870]), available in the [SW|Jörg Stülke] lab

References

2509422, 3923430, 3130545, 2509423, 21661762, 20933603, 15378759, 6098632

2509422, 3923430, 3130545, 2509423, 20933603, 15378759, 6098632, 8909293, 29140245

Expression and Regulation

additional information

the protein amounts increase upon DNA damage (MMS treatment) [pubmed|29140245]

Biological materials

Expression vectors

pGP1122 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab) [pubmed|20933603]