locus

BSU13420

BSU_13420

outlinks

bsu

BSU13420

BSU_13420

The protein

[SW|Domains]

contains a N-terminal [SW|PAS domain] [Pubmed|23893111]

contains a central [SW|GGDEF domain] and a C-terminal [SW|EAL domain] [Pubmed|22821967]

aa 290-735 are similar to ''E. coli'' CsrD (18% identity, 43% similarity)

MHYT domain (aa 7-201) (according to UniProt)

contains a N-terminal [SW|PAS domain] [Pubmed|23893111]

contains a central [SW|GGDEF domain] and a C-terminal [SW|EAL domain] [Pubmed|22821967]

aa 290-735 are similar to ''E. coli'' CsrD (18% identity, 43% similarity)

Biological materials

Mutant

MGNA-B314 (ykoW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1313 NBRP B. subtilis, Japan]

GP850 (''[[gene|dgcW]]''::''cat''), available in [SW|Jrg Stlke]'s lab [pubmed|28420751]

BP140 (''[[gene|dgcW]]''::''ermC'') available in [SW|Fabian Commichau]'s lab

BP142 (''[[gene|dgcW]]-[[gene|ykoV]]-[[gene|ligD]]''::''kan'') available in [SW|Fabian Commichau]'s lab

BKE13420 ([[gene|dgcW]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGGAGATTCCCCCCGT, downstream forward: _UP4_TAACAGCGCCGGCTTTTTTT

BKK13420 ([[gene|dgcW]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGGAGATTCCCCCCGT, downstream forward: _UP4_TAACAGCGCCGGCTTTTTTT

MGNA-B314 (ykoW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1313 NBRP B. subtilis, Japan]

GP850 (''[[gene|dgcW]]''::''cat''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

BP140 (''[[gene|dgcW]]''::''ermC'') available in [SW|Fabian Commichau]'s lab

BP142 (''[[gene|dgcW]]-[[gene|ykoV]]-[[gene|ligD]]''::''kan'') available in [SW|Fabian Commichau]'s lab

BKE13420 ([[gene|dgcW]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGGAGATTCCCCCCGT, downstream forward: _UP4_TAACAGCGCCGGCTTTTTTT

BKK13420 ([[gene|dgcW]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGGAGATTCCCCCCGT, downstream forward: _UP4_TAACAGCGCCGGCTTTTTTT

Biological materials

two-hybrid system

B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]'s lab

B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab