SubtiBank SubtiBank
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2017-07-07 10:44:352025-05-10 17:13:59

description

high affinity potassium transporter [[protein|KtrA]]-[[protein|KtrB]], integral membrane subunit

high affinity potassium channel [[protein|KtrA]]-[[protein|KtrB]], integral membrane subunit

locus

BSU31100

BSU_31100

geneLength

1335

1338

product

high affinity potassium transporter [[protein|KtrA]]-[[protein|KtrB]], integral membrane subunit

high affinity potassium channel [[protein|KtrA]]-[[protein|KtrB]], integral membrane subunit

outlinks

bsu

BSU31100

BSU_31100

Gene

Coordinates

3,189,089 → 3,190,426

3,189,089 3,190,426

The protein

Paralogous protein(s)

[[protein|KtrD]]

[[this]]

Biological materials

Mutant

MGNA-A227 (yubG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/227 NBRP B. subtilis, Japan]

1A954 ( ''ktrB''::''kan''), [Pubmed|12562800], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A954&Search=1A954 BGSC]

GHB1 (D(''[[gene|ktrA]]-[[gene|ktrB]]'')::''aphA3''), available in [SW|Erhard Bremer]'s lab

GP92 (D(''[[gene|ktrA]]-[[gene|ktrB]]'')::''aphA3''), available in [SW|Jörg Stülke]'s lab

MGNA-A227 (yubG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/227 NBRP B. subtilis, Japan]

1A954 ( ''ktrB''::''kan''), [Pubmed|12562800], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A954&Search=1A954 BGSC]

GHB1 ([[gene|ktrA]]-[[gene|ktrB]]::''aphA3''), available in [SW|Erhard Bremer]'s lab

GP92 ([[gene|ktrA]]-[[gene|ktrB]]::''aphA3''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

GP2083 ([[gene|ktrA]]-[[gene|ktrB]]::''aphA3'' [[gene|ktrC]]::''tet''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

GP2498 ([[gene|ktrA]]-[[gene|ktrB]]::''spc'' [[gene|kimA]]::''cat''), available in [SW|Jörg Stülke]'s lab

BKE31100 ([[gene|ktrB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTTTGTCTACATCCCTT, downstream forward: _UP4_TGATATCAAAAAAATCCGGC

BKK31100 ([[gene|ktrB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTTTGTCTACATCCCTT, downstream forward: _UP4_TGATATCAAAAAAATCCGGC

GP2716 ([[gene|ktrA]]-[[gene|ktrB]]::''spc''), available in [SW|Jörg Stülke]'s lab

GP3064 ([[gene|ktrB]]::''kan''), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

[SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

References

Reviews

25869574, 21680052, 27935846, 25838295

25869574, 21680052, 27935846, 25838295, 28825218

References

Original publications

12562800, 20511502, 17932047, 23086297, 23598340, 16990138, 24141192, 26771197, 28420751, 28504641, 28679749

12562800, 20511502, 17932047, 23086297, 23598340, 16990138, 24141192, 26771197, 28420751, 28504641, 28679749, 30753894, 31624134, 32253343

The protein

Catalyzed reaction/ biological activity

uptake of potassium

The protein

Protein family

TrkH potassium transport family (with [[protein|KtrD]], according to UniProt)

The protein

Kinetic information

the [[protein|KtrA]]-[[protein|KtrB]] channel has a high affinity for potassium,this is determined by [[protein|KtrB]] [pubmed|30753894]

Expression and Regulation

additional information

growth at extreme potassium limitation results in the acquisition of promoter mutations with increased [[gene|ktrA]]-[[gene|ktrB]] expression [pubmed|28679749]

Biological materials

Expression vector

pGP2992: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

Biological materials

FLAG-tag construct

GP2277 (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab [pubmed|28679749]

labs

[SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi Homepage]

[SW|Inga Hänelt], Frankfurt, Germany [https://www.biochem.uni-frankfurt.de/index.php?id=298 Homepage]

[SW|João H Morais-Cabral], University of Porto, Portugal [https://www.i3s.up.pt/research-group?x=47 Homepage]

[SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]