description

"Carbon-flux regulating HPr", formerly known as "Catabolite repression HPr-like protein", control of flux through the harmful methylglyoxal pathway, minor cofactor of the [[protein|CcpA ]]transcription factor

Carbon-flux regulating HPr, formerly known as Catabolite repression HPr-like protein, control of flux through the harmful methylglyoxal pathway, minor cofactor of the [[protein|CcpA]] transcription factor

locus

BSU34740

BSU_34740

geneLength

255

258

outlinks

bsu

BSU34740

BSU_34740

Gene

Coordinates

3,569,292 → 3,569,549

3,569,292 3,569,549

The protein

Protein family

[[protein|PtsH|HPr]] family

HPr family (with [[protein|PtsH]], according to UniProt)

The protein

[SW|Domains]

[[protein|PtsH|HPr]] domain (1–85)

Hpr domain (aa 1-85) (according to UniProt)

The protein

Structure

[PDB|2AK7] (dimeric Crh-Ser46-P)

[PDB|1ZVV] ([[protein|CcpA]]-Crh-DNA complex)

[PDB|2RLZ] (dimer)

[PDB|1MU4]

[http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=24634 NCBI], dimer

[http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=64943 NCBI], [[protein|CcpA]]-Crh-DNA complex

[http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=52326 NCBI], dimeric phosphor-Crh

[http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=39567 NCBI]

[PDB|2AK7] (dimeric Crh-Ser46-P) [pubmed|16411239]

[PDB|1ZVV] ([[protein|CcpA]]-Crh-DNA complex)

[PDB|2RLZ] (dimer)

[PDB|1MU4]

Biological materials

Mutant

GP860 (aphA3) [Pubmed|21992469], QB7097 (spc), available in [SW|Jörg Stülke]'s lab

BKE34740 (Δ[[gene|crh]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGATCTCCCCTTTTCT, downstream forward: _UP4_GCTTACGTTCAAGAAGAAGT

BKK34740 (Δ[[gene|crh]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGATCTCCCCTTTTCT, downstream forward: _UP4_GCTTACGTTCAAGAAGAAGT

GP860 (Δ[[gene|crh]]::aphA3) [Pubmed|21992469], available in [SW|Jörg Stülke]'s lab

QB7097 (spc), available in [SW|Jörg Stülke]'s lab

BKE34740 (Δ[[gene|crh]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGATCTCCCCTTTTCT, downstream forward: _UP4_GCTTACGTTCAAGAAGAAGT

BKK34740 (Δ[[gene|crh]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGATCTCCCCTTTTCT, downstream forward: _UP4_GCTTACGTTCAAGAAGAAGT

Biological materials

Expression vector

pGP641 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab

pGP734 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

[SW|Boris Görke], University of Göttingen, Germany

[http://wwwuser.gwdg.de/~genmibio/goerke.html Homepage]

[SW|Anne Galinier], University of Marseille, France

[SW|Wolfgang Hillen], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]

[SW|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]

References

12972249, 9973552, 9237995, 16272399, 15126459, 10217795, 17142398, 16316990, 12009882, 10589728, 12670692, 11916384, 11361076, 11361074, 18320329, 18284240, 16411239, 21992469, 22092971

12972249, 9973552, 9237995, 16272399, 15126459, 10217795, 17142398, 16316990, 12009882, 10589728, 12670692, 11916384, 11361076, 11361074, 18320329, 18284240, 16411239, 21992469, 22092971, 29514981

Biological materials

Expression vectors

pGP641 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab

pGP734 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab

labs

[SW|Anne Galinier], University of Marseille, France

[SW|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]