2017-8-11 16:0:32025-05-23 11:55:09
_ec
description
low affinity potassium transporter [[protein|KtrC]]-[[protein|KtrD]], integral membrane subunit
glutamate-controlled potassium channel [[protein|KtrC]]-[[protein|KtrD]], integral membrane subunit
locus
BSU13500
BSU_13500
geneLength
1347
1350
product
low affinity potassium transporter [[protein|KtrC]]-[[protein|KtrD]], integral membrane subunit (proton symport)
glutamate-controlled potassium channel [[protein|KtrC]]-[[protein|KtrD]], integral membrane subunit
outlinks
bsu
BSU13500
BSU_13500
Gene
Coordinates
1,416,067 → 1,417,416
1,416,067 1,417,416
The protein
Protein family
View classification (according to Swiss-Prot)
TrkH potassium transport family (with [[protein|KtrB]], according to UniProt)
The protein
Paralogous protein(s)
[[protein|KtrB]]
[[this]]
Biological materials
Mutant
MGNA-B317 (ykrM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1316 NBRP B. subtilis, Japan]
GHB12 (''ktrD''::''tet''), [Pubmed|12562800], available in [SW|Erhard Bremer]'s and [SW|Jörg Stülke]'s labs
GP2030 (''ktrD''::''tet''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]
1A956 (''ktrD''::''tet''), [Pubmed|12562800], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A956&Search=1A956 BGSC]
MGNA-B317 (ykrM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1316 NBRP B. subtilis, Japan]
GHB12 (''ktrD''::''tet''), [Pubmed|12562800], available in [SW|Erhard Bremer]'s and [SW|Jörg Stülke]'s labs
GP2030 (''ktrD''::''tet''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]
1A956 (''ktrD''::''tet''), [Pubmed|12562800], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A956&Search=1A956 BGSC]
BKE13500 ([[gene|ktrD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAATCCTTCTGCCT, downstream forward: _UP4_TAGGCAAAACACCGCATATT
BKK13500 ([[gene|ktrD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAATCCTTCTGCCT, downstream forward: _UP4_TAGGCAAAACACCGCATATT
Biological materials
FLAG-tag construct
GP2434 ''ktrD-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
GP3465 ''ktrD-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
Labs working on this gene/protein
[SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]
References
Original publications
The protein
Catalyzed reaction/ biological activity
uptake of potassium
The protein
Kinetic information
the [[protein|KtrC]]-[[protein|KtrD ]]channel has a low affinity for potassium,this is determined by KtrD [pubmed|30753894]
the affinity of [[protein|KtrC]]-[[protein|KtrD]] for potassium is strongly increased in the presence of glutamate (from 0.278 mM to 0.17 mM) [pubmed|32253343]
The protein
Effectors of protein activity
the affinity of [[protein|KtrC]]-[[protein|KtrD]] for potassium is strongly increased in the presence of glutamate [pubmed|32253343]
Biological materials
Expression vector
pGP2996: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
labs
[SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi Homepage]
[SW|Inga Hänelt], Frankfurt, Germany [https://www.biochem.uni-frankfurt.de/index.php?id=298 Homepage]
[SW|João H Morais-Cabral], University of Porto, Portugal [https://www.i3s.up.pt/research-group?x=47 Homepage]
[SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]