Tue Dec 13 2016 11:16:34 GMT+0100 (CET)2025-05-12 01:28:21
locus
BSU24040
BSU_24040
outlinks
bsu
BSU24040
BSU_24040
aminos
MSVMSYIDAINLAMKEEMERDSRVFVLGEDVGRKGGVFKATAGLYEQFGEERVMDTPLAESAIAGVGIGAAMYGMRPIAEMQFADFIMPAVNQIISEAAKIRYRSNNDWSCPIVVRAPYGGGVHGALYHSQSVEAIFANQPGLKIVMPSTPYDAKGLLKAAVRDEDPVLFFEHKRAYRLIKGEVPADDYVLPIGKADVKREGDDITVITYGLCVHFALQAAERLEKDGISAHVVDLRTVYPLDKEAIIEAASKTGKVLLVTEDTKEGSIMSEVAAIISEHCLFDLDAPIKRLAGPDIPAMPYAPTMEKYFMVNPDKVEAAMRELAEF
[SW|Categories] containing this gene/protein
[SW|utilization of amino acids]
Gene
Coordinates on the chromosome (coding sequence)
2,498,093 -> 2,499,076
The protein
Catalyzed reaction/ biological activity
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2 (according to Swiss-Prot)
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase]-(R)-N6-lipoyl-L-lysine + H+ --> [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase]-(R)-N6-(S8-2-methylpropanoyldihydrolipoyl)-L-lysine + CO2 (according to UniProt)
The protein
Paralogous protein(s)
[[protein|PdhB]], [[protein|AcoB]]
[[this]]
Expression and Regulation
Operon
''[[protein|ptb]]-[[protein|bcd]]-[[protein|buk]]-[[protein|lpdV]]-[SW|bkdAA]-[SW|bkdAB]-[[protein|bkdB]]'' [Pubmed|12823818]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigL]] [Pubmed|10094682]
Expression and Regulation
Regulation
induced in the presence of isoleucine or valine ([[protein|BkdR]]) [Pubmed|10094682]
Expression and Regulation
Regulatory mechanism
[[protein|CodY]]: transcription repression [Pubmed|10094682]
[[protein|BkdR]]: transcription activation [Pubmed|10094682]
References
proteinLength
327
geneLength
984
Gene
Coordinates
2,498,093 2,499,076
The protein
Structure
[PDB|3DUF] (Geobacillus stearothermophilus [[protein|PdhC]], 36% identity) [pubmed|19081062]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-C437 (bfmBAB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2435 NBRP B. subtilis, Japan]
BKE24040 ([[gene|bkdAB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACTGACATTTGTTCTT, downstream forward: _UP4_TAAAGACGTAAGGGAGGATA
BKK24040 ([[gene|bkdAB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACTGACATTTGTTCTT, downstream forward: _UP4_TAAAGACGTAAGGGAGGATA