SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:34 GMT+0100 (CET)2025-05-12 01:28:21

locus

BSU24040

BSU_24040

outlinks

bsu

BSU24040

BSU_24040

aminos

MSVMSYIDAINLAMKEEMERDSRVFVLGEDVGRKGGVFKATAGLYEQFGEERVMDTPLAESAIAGVGIGAAMYGMRPIAEMQFADFIMPAVNQIISEAAKIRYRSNNDWSCPIVVRAPYGGGVHGALYHSQSVEAIFANQPGLKIVMPSTPYDAKGLLKAAVRDEDPVLFFEHKRAYRLIKGEVPADDYVLPIGKADVKREGDDITVITYGLCVHFALQAAERLEKDGISAHVVDLRTVYPLDKEAIIEAASKTGKVLLVTEDTKEGSIMSEVAAIISEHCLFDLDAPIKRLAGPDIPAMPYAPTMEKYFMVNPDKVEAAMRELAEF

[SW|Categories] containing this gene/protein

[SW|utilization of amino acids]

Gene

Coordinates on the chromosome (coding sequence)

2,498,093 -> 2,499,076

The protein

Catalyzed reaction/ biological activity

3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2 (according to Swiss-Prot)

3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase]-(R)-N6-lipoyl-L-lysine + H+ --> [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase]-(R)-N6-(S8-2-methylpropanoyldihydrolipoyl)-L-lysine + CO2 (according to UniProt)

The protein

Paralogous protein(s)

[[protein|PdhB]], [[protein|AcoB]]

[[this]]

Expression and Regulation

Operon

''[[protein|ptb]]-[[protein|bcd]]-[[protein|buk]]-[[protein|lpdV]]-[SW|bkdAA]-[SW|bkdAB]-[[protein|bkdB]]'' [Pubmed|12823818]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigL]] [Pubmed|10094682]

Expression and Regulation

Regulation

induced in the presence of isoleucine or valine ([[protein|BkdR]]) [Pubmed|10094682]

Expression and Regulation

Regulatory mechanism

[[protein|CodY]]: transcription repression [Pubmed|10094682]

[[protein|BkdR]]: transcription activation [Pubmed|10094682]

References

12427936, 15241682, 10094682, 12823818, 16479537, 1886522

12427936, 15241682, 10094682, 12823818, 16479537, 1886522, 19081062

proteinLength

327

geneLength

984

Gene

Coordinates

2,498,093 2,499,076

The protein

Structure

[PDB|3DUF] (Geobacillus stearothermophilus [[protein|PdhC]], 36% identity) [pubmed|19081062]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-C437 (bfmBAB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2435 NBRP B. subtilis, Japan]

BKE24040 ([[gene|bkdAB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACTGACATTTGTTCTT, downstream forward: _UP4_TAAAGACGTAAGGGAGGATA

BKK24040 ([[gene|bkdAB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACTGACATTTGTTCTT, downstream forward: _UP4_TAAAGACGTAAGGGAGGATA