Tue Dec 13 2016 11:16:31 GMT+0100 (CET)2025-05-19 09:57:53
description
NAD(P)H-flavin nitroreductase
NADPH-flavin nitroreductase
locus
BSU07830
BSU_07830
product
NAD(P)H-flavin nitroreductase
NADPH-flavin nitroreductase
ec
outlinks
bsu
BSU07830
BSU_07830
aminos
MADLKTQILDAYNFRHATKEFDPNKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGAQKQLPTASHFVLILARTAKDIKYNADYIKRHLKEVKQMPQDVYEGYLSKTEEFQKNDLHLLESDRTLFDWASKQTYIALGNMMTAAAQIGVDSCPIEGFQYDHIHRILEEEGLLENGSFDISVMVAFGYRVRDPRPKTRSAVEDVVKWV
[SW|Categories] containing this gene/protein
[SW|electron transport/ other], [SW|resistance against oxidative and electrophile stress]
Gene
Coordinates on the chromosome (coding sequence)
854,412 -> 855,077
Gene
Phenotypes of a mutant
a ''[[protein|hypO]] [[protein|ohrA]]'' mutant is more sensitive to NaOCl stress than ''[[protein|ohrA]]'' single mutant [Pubmed|22238377]
a ''[[gene|hypO]] [[gene|ohrA]]'' mutant is more sensitive to NaOCl stress than ''[[gene|ohrA]]'' single mutant [Pubmed|22238377]
The protein
Protein family
nitroreductase family (according to Swiss-Prot)
[SW|nitroreductase family] (according to UniProt)
The protein
[SW|Cofactors]
FMN or FAD [Pubmed|21635694]
FMN [Pubmed|21635694] (according to UniProt)
NADP [pubmed|31392514]
Expression and Regulation
Operon
''hypO'' [Pubmed|22238377]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|22238377]
Expression and Regulation
Regulation
induced under disulfide stress conditions (diamide, NaOCl) ([[protein|HypR]]) [Pubmed|22238377]
Expression and Regulation
Regulatory mechanism
[[protein|HypR]]:transcription activation [Pubmed|22238377]
Labs working on this gene/protein
[SW|Haike Antelmann], University of Greifswald, Germany
References
Original articles
proteinLength
221
geneLength
666
Gene
Coordinates
854,412 855,077
The protein
Catalyzed reaction/ biological activity
reduction of 2,6-dinitrotoluene [pubmed|31392514]
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|2H0U] (from Helicobacter pylori, 45% identity)
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-C366 (yfkO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2364 NBRP B. subtilis, Japan]
BKE07830 ([[gene|hypO]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAACACCTCTCCTT, downstream forward: _UP4_TAAAAGAAGAAAGCTGCTCG
BKK07830 ([[gene|hypO]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAACACCTCTCCTT, downstream forward: _UP4_TAAAAGAAGAAAGCTGCTCG
labs
[SW|Haike Antelmann], University of Greifswald, Germany
References
Original publications