SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:36 GMT+0100 (CET)2025-05-25 07:40:38

locus

BSU33090

BSU_33090

function

control of LiaR activity in response to bacitracin

control of [[protein|LiaR]] activity in response to bacitracin

ec

outlinks

bsu

BSU33090

BSU_33090

aminos

MRKKMLASLQWRAIRMTTGISLLLFVCLISFMMFYYRLDPLVLLSSSWFGIPFILILLLISVTVGFASGYMYGNRLKTRIDTLIESILTFENGNFAYRIPPLGDDEIGLAADQLNEMAKRVELQVASLQKLSNERAEWQAQMKKSVISEERQRLARDLHDAVSQQLFAISMMTSAVLEHVKDADDKTVKRIRMVEHMAGEAQNEMRALLLHLRPVTLEGKGLKEGLTELLDEFRKKQPIDIEWDIQDTAISKGVEDHLFRIVQEALSNVFRHSKASKVTVILGIKNSQLRLKVIDNGKGFKMDQVKASSYGLNSMKERASEIGGVAEVISVEGKGTQIEVKVPIFPEEKGENERDSSIID

[SW|Categories] containing this gene/protein

[SW|protein modification], [SW|transcription factors and their control], [SW|resistance against oxidative and electrophile stress], [SW|resistance against toxins/ antibiotics], [SW|membrane proteins], [SW|phosphoproteins]

Gene

Coordinates on the chromosome (coding sequence)

3,395,035 -> 3,396,117

The protein

Catalyzed reaction/ biological activity

autophosphorylation, phosphorylation of [[protein|LiaR]]

dephosphorylation of [[protein|LiaR]] in the absence of the stress signal [Pubmed|23279150]

autophosphorylation, phosphorylation of [[protein|LiaR]]

dephosphorylation of [[protein|LiaR]] in the absence of the stress signal [Pubmed|23279150]

ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

The protein

[SW|Domains]

two transmembrane segments, C-terminal histidine phosphotransferase domain

two transmembrane segments

[SW|HAMP domain] (aa 74-126) (according to UniProt)

[SW|Histidine kinase domain] (aa 153-346) (according to UniProt)

The protein

[SW|Localization]

cell membrane (according to Swiss-Prot)

cell membrane (according to UniProt)

The protein

[SW|Interactions]

[[protein|LiaS]]-[[protein|LiaR]]

Expression and Regulation

Operon

''[[protein|liaI]]-[[protein|liaH]]-[[protein|liaG]]-[[protein|liaF]]-[[protein|liaS]]-[[protein|liaR]]'' [Pubmed|14651641]

''[[protein|liaG]]-[[protein|liaF]]-[[protein|liaS]]-[[protein|liaR]]'' [Pubmed|16816187]

Expression and Regulation

[SW|Sigma factor]

''liaI'': [[protein|SigA]] [Pubmed|15273097]

''liaG'': [[protein|SigA]] [Pubmed|16816187]

Expression and Regulation

Regulation

''[[protein|liaI]]'': induced by bacitracin and rhamnolipids ([[protein|LiaR]]) [Pubmed|16816187,22092710]

''[[protein|liaI]]'': repressed during logarithmic growth ([[protein|AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15101989,17660417 PubMed], indirect regulation by [[protein|AbrB]] [Pubmed|20817675]

''[[protein|liaG]]'': constitutive

Expression and Regulation

Regulatory mechanism

[[protein|LiaR]]: transcription activation [Pubmed|16816187]

Labs working on this gene/protein

[SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]

References

Original publications

18394148, 19164152, 15273097, 17921301, 10094672, 19164157, 14651641, 17600057, 17660417, 16816187, 15101989, 17660417, 20057163, 23279150, 20817675, 22964256, 22092710, 23326432, 27856233

18394148, 19164152, 15273097, 17921301, 10094672, 19164157, 14651641, 17600057, 17660417, 16816187, 15101989, 17660417, 20057163, 23279150, 20817675, 22964256, 22092710, 23326432, 27856233, 29185696, 19805278, 30848888, 32117169, 32349670, 33114184

proteinLength

360

geneLength

1083

Gene

Coordinates

3,395,035 3,396,117

The protein

Paralogous protein(s)

[[this]]

The protein

Structure

[PDB|3GIG] ([[protein|DesK]], corresponds to the C-terminal domain, aa 149 ... 343, 25% identity) [pubmed|19805278]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-B036 (yvqE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1035 NBRP B. subtilis, Japan]

BKE33090 ([[gene|liaS]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAGGCTGGCAAGCATTTTTT, downstream forward: _UP4_CCGGAAGAAAAAGGAGAGAA

BKK33090 ([[gene|liaS]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAGGCTGGCAAGCATTTTTT, downstream forward: _UP4_CCGGAAGAAAAAGGAGAGAA

labs

[SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]