Tue Dec 13 2016 11:16:36 GMT+0100 (CET)2025-05-25 07:40:38
locus
BSU33090
BSU_33090
function
control of LiaR activity in response to bacitracin
control of [[protein|LiaR]] activity in response to bacitracin
ec
outlinks
bsu
BSU33090
BSU_33090
aminos
MRKKMLASLQWRAIRMTTGISLLLFVCLISFMMFYYRLDPLVLLSSSWFGIPFILILLLISVTVGFASGYMYGNRLKTRIDTLIESILTFENGNFAYRIPPLGDDEIGLAADQLNEMAKRVELQVASLQKLSNERAEWQAQMKKSVISEERQRLARDLHDAVSQQLFAISMMTSAVLEHVKDADDKTVKRIRMVEHMAGEAQNEMRALLLHLRPVTLEGKGLKEGLTELLDEFRKKQPIDIEWDIQDTAISKGVEDHLFRIVQEALSNVFRHSKASKVTVILGIKNSQLRLKVIDNGKGFKMDQVKASSYGLNSMKERASEIGGVAEVISVEGKGTQIEVKVPIFPEEKGENERDSSIID
[SW|Categories] containing this gene/protein
[SW|protein modification], [SW|transcription factors and their control], [SW|resistance against oxidative and electrophile stress], [SW|resistance against toxins/ antibiotics], [SW|membrane proteins], [SW|phosphoproteins]
Gene
Coordinates on the chromosome (coding sequence)
3,395,035 -> 3,396,117
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [[protein|LiaR]]
dephosphorylation of [[protein|LiaR]] in the absence of the stress signal [Pubmed|23279150]
autophosphorylation, phosphorylation of [[protein|LiaR]]
dephosphorylation of [[protein|LiaR]] in the absence of the stress signal [Pubmed|23279150]
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
The protein
[SW|Domains]
two transmembrane segments, C-terminal histidine phosphotransferase domain
two transmembrane segments
[SW|HAMP domain] (aa 74-126) (according to UniProt)
[SW|Histidine kinase domain] (aa 153-346) (according to UniProt)
The protein
[SW|Localization]
cell membrane (according to Swiss-Prot)
cell membrane (according to UniProt)
The protein
[SW|Interactions]
[[protein|LiaS]]-[[protein|LiaR]]
Expression and Regulation
Operon
''[[protein|liaI]]-[[protein|liaH]]-[[protein|liaG]]-[[protein|liaF]]-[[protein|liaS]]-[[protein|liaR]]'' [Pubmed|14651641]
''[[protein|liaG]]-[[protein|liaF]]-[[protein|liaS]]-[[protein|liaR]]'' [Pubmed|16816187]
Expression and Regulation
[SW|Sigma factor]
''liaI'': [[protein|SigA]] [Pubmed|15273097]
''liaG'': [[protein|SigA]] [Pubmed|16816187]
Expression and Regulation
Regulation
''[[protein|liaI]]'': induced by bacitracin and rhamnolipids ([[protein|LiaR]]) [Pubmed|16816187,22092710]
''[[protein|liaI]]'': repressed during logarithmic growth ([[protein|AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15101989,17660417 PubMed], indirect regulation by [[protein|AbrB]] [Pubmed|20817675]
''[[protein|liaG]]'': constitutive
Expression and Regulation
Regulatory mechanism
[[protein|LiaR]]: transcription activation [Pubmed|16816187]
Labs working on this gene/protein
[SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
References
Original publications
proteinLength
360
geneLength
1083
Gene
Coordinates
3,395,035 3,396,117
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|3GIG] ([[protein|DesK]], corresponds to the C-terminal domain, aa 149 ... 343, 25% identity) [pubmed|19805278]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-B036 (yvqE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1035 NBRP B. subtilis, Japan]
BKE33090 ([[gene|liaS]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAGGCTGGCAAGCATTTTTT, downstream forward: _UP4_CCGGAAGAAAAAGGAGAGAA
BKK33090 ([[gene|liaS]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAGGCTGGCAAGCATTTTTT, downstream forward: _UP4_CCGGAAGAAAAAGGAGAGAA
labs
[SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]