Tue Dec 13 2016 11:16:33 GMT+0100 (CET)2025-05-30 17:41:44
title
ylnE
sirB
description
probable siroheme ferrochelatase
sirohydrochlorin ferrochelatase
locus
BSU15620
BSU_15620
product
probable siroheme ferrochelatase
sirohydrochlorin ferrochelatase
ec
names
ylnE
sirB
ylnE
outlinks
bsu
BSU15620
BSU_15620
aminos
MKQAILYVGHGSRVKKAQQEAAAFLEGCKAHISVPVQEISFLELQEPTIETGFEACVKQGATHIAVVPLLLLTAAHAKHDIPEEIVRVASRYPSVRISYGKPIGIDEEVVKAVYHRMKDIGVPYENARVVLIGRGSSDPDVKRDVTGIANLLQEMVPVKEVIPCFLTACGPNYKEVFSELEKDDGITTFIVPYLLFTGMLMNEIEREVQKLKAHNPNVYLSSYIGFHPHVKNAFLNRVRETAANSEGQFDFDGGSYASAAH
[SW|Categories] containing this gene/protein
[SW|biosynthesis of cofactors]
Gene
Coordinates on the chromosome (coding sequence)
1,634,837 -> 1,635,622
The protein
Catalyzed reaction/ biological activity
Siroheme + 2 H+ = sirohydrochlorin + Fe2+ (according to Swiss-Prot)
insertion of Fe2+ into the substrate sirohydrochlorin (SHC) in siroheme biosynthesis [pubmed|30778451]
2 H+ + siroheme --> Fe2+ + sirohydrochlorin (according to UniProt)
The protein
Protein family
SirB subfamily (according to Swiss-Prot)
CbiX family (single member, according to UniProt)
Expression and Regulation
Operon
''[[protein|cysH]]-[[protein|cysP]]-[[protein|sat]]-[[protein|cysC]]-[[protein|ylnD]]-[[protein|ylnE]]-[[protein|ylnF]]'' [Pubmed|11004190]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|11004190]
Expression and Regulation
Regulation
repressed by casamino acids [Pubmed|12107147]
repressed in the presence of cysteine ([[protein|CymR]])
induced by methionine starvation ([[protein|S-box]]) [Pubmed|10094622]
Expression and Regulation
Regulatory mechanism
[[protein|S-box]]: transcription termination/ antitermination, the [[protein|S-box]] [SW|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622]
[[protein|CymR]]: transcription repression
References
proteinLength
261
geneLength
786
Gene
Coordinates
1,634,837 1,635,622
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
Apo-form: [PDB|5ZT8] [pubmed|30778451]
Co2+ bound: [PDB|5ZT7] ([pubmed|30778451]), [PDB|6JV6]
Ni2+ bound: [PDB|5ZT9]
Fe3+ bound: [PDB|5ZTA]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-B369 (ylnE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1368 NBRP B. subtilis, Japan]
BKE15620 ([[gene|sirB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATATAAAATTGCTTGTTTCA, downstream forward: _UP4_CAATTTGATTTTGATGGAGG
BKK15620 ([[gene|sirB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATATAAAATTGCTTGTTTCA, downstream forward: _UP4_CAATTTGATTTTGATGGAGG