SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:34 GMT+0100 (CET)2025-05-13 20:04:00

locus

BSU19430

BSU_19430

outlinks

bsu

BSU19430

BSU_19430

aminos

MKAMRYEQISENAFKGKIQVYLEQILGDASLILKTLHEKDQCLLCELDDLGHVFQDMQGIASSFYLQSYIEEFTPAFIELAKAIKALSEHKHGALIVIERADPVERFIQKGTSLHAEISSSLIESIFFPGNPLHDGALLVRENKLVSAANVLPLTTKEVDIHLGTRHRAALGMSGYTDALVLVVSEETGKMSFAKDGVLYPLISPRT

[SW|Categories] containing this gene/protein

[SW|metabolism of signalling nucleotides], [SW|sporulation proteins]

Gene

Coordinates on the chromosome (coding sequence)

2,118,504 -> 2,119,127

The protein

Catalyzed reaction/ biological activity

synthesis of c-di-AMP from two molecules of ATP [Pubmed|23192352]

synthesis of c-di-AMP from two molecules of ATP [Pubmed|23192352]

2 ATP --> 3',3'-c-di-AMP + 2 diphosphate (according to UniProt)

The protein

Paralogous protein(s)

[[protein|CdaA]]

[[this]]

The protein

[SW|Domains]

N-terminal autoinhibitory domain [Pubmed|24939848]

C-terminal [SW|DAC domain] involved in the synthesis of c-di-AMP [Pubmed|21566650]

N-terminal autoinhibitory domain [Pubmed|24939848]

C-terminal [SW|DAC domain] involved in the synthesis of c-di-AMP [Pubmed|21566650]

[SW|DAC domain] (aa 63-205) (according to UniProt)

The protein

[SW|Interactions]

forms hexamers (enzymatically inactive) or dimers (active) [Pubmed|24939848]

Expression and Regulation

Operon

''[[protein|cdaS]]'' [Pubmed|22383849]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigG]] [Pubmed|22383849]

Expression and Regulation

Regulation

expressed late during sporulation in the forespore ([[protein|SigG]]) [Pubmed|22383849]

Biological materials

Mutant

GP983 (''[[protein|cdaS]]''::''ermC''), available in [SW|Jörg Stülke]'s lab

GP1360 (''[[protein|cdaS]]''::''spc''), available in [SW|Jörg Stülke]'s lab

MGNA-B426 (yojJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1425 NBRP B. subtilis, Japan]

GP983 (''[[gene|cdaS]]''::''ermC''), available in [SW|Jörg Stülke]'s lab [pubmed|23192352]

GP1360 (''[[gene|cdaS]]''::''spc''), available in [SW|Jörg Stülke]'s lab

GP2222 ([[gene|cdaA]]::cat [[gene|cdaS]]::ermC ''[[gene|disA]]''::''tet''), available in [SW|Jörg Stülke]'s lab, the mutant is only viable on minimal medium at low potassium concentration [pubmed|28420751]

BKE19430 ([[gene|cdaS]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCCTGTTTAAATTTCC, downstream forward: _UP4_TAAAAATAATAAAAAGGGCC

BKK19430 ([[gene|cdaS]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCCTGTTTAAATTTCC, downstream forward: _UP4_TAAAAATAATAAAAAGGGCC

Biological materials

Expression vector

IPTG inducible expression of '''''B. cereus''''' Strep-''cdaS'' in ''E. coli'': pGP2593 (in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

References

Reviews

25869574, 18714086, 23812326

25869574, 18714086, 23812326, 30224435, 32472931, 32603625

proteinLength

207

geneLength

624

Gene

Coordinates

2,118,504 2,119,127

Gene

Phenotypes of a mutant

a [[gene|cdaA]] [[gene|cdaS]] [[gene|disA]] triple mutant is not viable on complex medium; however, the mutant grows at low potassium concentration (0.1 mM) [pubmed|28420751]

The protein

Protein family

adenylate cyclase family (with [[protein|CdaA]], according to UniProt)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Expression vectors

IPTG inducible expression of '''''B. cereus''''' Strep-''cdaS'' in ''E. coli'': pGP2593 (in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

labs

[SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]