Tue Dec 13 2016 11:16:34 GMT+0100 (CET)2025-05-13 20:04:00
locus
BSU19430
BSU_19430
outlinks
bsu
BSU19430
BSU_19430
aminos
MKAMRYEQISENAFKGKIQVYLEQILGDASLILKTLHEKDQCLLCELDDLGHVFQDMQGIASSFYLQSYIEEFTPAFIELAKAIKALSEHKHGALIVIERADPVERFIQKGTSLHAEISSSLIESIFFPGNPLHDGALLVRENKLVSAANVLPLTTKEVDIHLGTRHRAALGMSGYTDALVLVVSEETGKMSFAKDGVLYPLISPRT
[SW|Categories] containing this gene/protein
[SW|metabolism of signalling nucleotides], [SW|sporulation proteins]
Gene
Coordinates on the chromosome (coding sequence)
2,118,504 -> 2,119,127
The protein
Catalyzed reaction/ biological activity
synthesis of c-di-AMP from two molecules of ATP [Pubmed|23192352]
synthesis of c-di-AMP from two molecules of ATP [Pubmed|23192352]
2 ATP --> 3',3'-c-di-AMP + 2 diphosphate (according to UniProt)
The protein
Paralogous protein(s)
[[protein|CdaA]]
[[this]]
The protein
[SW|Domains]
N-terminal autoinhibitory domain [Pubmed|24939848]
C-terminal [SW|DAC domain] involved in the synthesis of c-di-AMP [Pubmed|21566650]
N-terminal autoinhibitory domain [Pubmed|24939848]
C-terminal [SW|DAC domain] involved in the synthesis of c-di-AMP [Pubmed|21566650]
[SW|DAC domain] (aa 63-205) (according to UniProt)
The protein
[SW|Interactions]
forms hexamers (enzymatically inactive) or dimers (active) [Pubmed|24939848]
Expression and Regulation
Operon
''[[protein|cdaS]]'' [Pubmed|22383849]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigG]] [Pubmed|22383849]
Expression and Regulation
Regulation
expressed late during sporulation in the forespore ([[protein|SigG]]) [Pubmed|22383849]
Biological materials
Mutant
GP983 (''[[protein|cdaS]]''::''ermC''), available in [SW|Jörg Stülke]'s lab
GP1360 (''[[protein|cdaS]]''::''spc''), available in [SW|Jörg Stülke]'s lab
MGNA-B426 (yojJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1425 NBRP B. subtilis, Japan]
GP983 (''[[gene|cdaS]]''::''ermC''), available in [SW|Jörg Stülke]'s lab [pubmed|23192352]
GP1360 (''[[gene|cdaS]]''::''spc''), available in [SW|Jörg Stülke]'s lab
GP2222 ([[gene|cdaA]]::cat [[gene|cdaS]]::ermC ''[[gene|disA]]''::''tet''), available in [SW|Jörg Stülke]'s lab, the mutant is only viable on minimal medium at low potassium concentration [pubmed|28420751]
BKE19430 ([[gene|cdaS]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCCTGTTTAAATTTCC, downstream forward: _UP4_TAAAAATAATAAAAAGGGCC
BKK19430 ([[gene|cdaS]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCCTGTTTAAATTTCC, downstream forward: _UP4_TAAAAATAATAAAAAGGGCC
Biological materials
Expression vector
IPTG inducible expression of '''''B. cereus''''' Strep-''cdaS'' in ''E. coli'': pGP2593 (in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
References
Reviews
proteinLength
207
geneLength
624
Gene
Coordinates
2,118,504 2,119,127
Gene
Phenotypes of a mutant
a [[gene|cdaA]] [[gene|cdaS]] [[gene|disA]] triple mutant is not viable on complex medium; however, the mutant grows at low potassium concentration (0.1 mM) [pubmed|28420751]
The protein
Protein family
adenylate cyclase family (with [[protein|CdaA]], according to UniProt)
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Expression vectors
IPTG inducible expression of '''''B. cereus''''' Strep-''cdaS'' in ''E. coli'': pGP2593 (in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
labs
[SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]