Tue Dec 13 2016 11:16:32 GMT+0100 (CET)2025-05-31 23:54:43
locus
BSU13600
BSU_13600
product
2-hydroxy-3-keto-5-methylthiopentenyl-
1-phosphate phosphatase
2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase
ec
outlinks
bsu
BSU13600
BSU_13600
aminos
MTTRKPFIICDFDGTITMNDNIINIMKTFAPPEWMALKDGVLSKTLSIKEGVGRMFGLLPSSLKEEITSFVLEDAKIREGFREFVAFINEHEIPFYVISGGMDFFVYPLLEGIVEKDRIYCNHASFDNDYIHIDWPHSCKGTCSNQCGCCKPSVIHELSEPNQYIIMIGDSVTDVEAAKLSDLCFARDYLLNECREQNLNHLPYQDFYEIRKEIENVKEVQEWLQNKNAGESSLK
[SW|Categories] containing this gene/protein
[SW|biosynthesis/ acquisition of amino acids]
Gene
Coordinates on the chromosome (coding sequence)
1,428,275 -> 1,428,982
The protein
Catalyzed reaction/ biological activity
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate
2-hydroxy-5-methylsulfanyl-3-oxopent-1-enyl phosphate + H2O --> 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate (according to UniProt)
The protein
Protein family
MtnX family (according to Swiss-Prot)
[SW|HAD superfamily]
[SW|HAD superfamily] (according to UniProt)
The protein
Structure
[PDB|2FEA]
[PDB|2FEA] [pubmed|17654724]
Expression and Regulation
Operon
''[[protein|mtnW]]-[[protein|mtnX]]-[[protein|mtnB]]-[[protein|mtnD]]'' [Pubmed|12022921]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|12022921]
Expression and Regulation
Regulation
repressed by casamino acids [Pubmed|12107147]
induced by methionine starvation ([[protein|S-box]]) [Pubmed|10094622]
Expression and Regulation
Regulatory mechanism
[[protein|S-box]]: transcription termination/ antitermination, the [[protein|S-box]] [SW|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622]
proteinLength
235
geneLength
708
Gene
Coordinates
1,428,275 1,428,982
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-A785 (ykrX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/785 NBRP B. subtilis, Japan]
BKE13600 ([[gene|mtnX]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATAATAAAAGGTTTTCGAG, downstream forward: _UP4_GAGAATGTAAAGGAGGTACA
BKK13600 ([[gene|mtnX]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATAATAAAAGGTTTTCGAG, downstream forward: _UP4_GAGAATGTAAAGGAGGTACA