2018-04-23 15:18:042025-04-28 12:07:11

description

MarR/DUF24 family transcription regulator, positively controls the nitroreductase gene hypO in response to disulfide stress

[SW|MarR family|MarR/DUF24 family] transcription regulator, positively controls the nitroreductase gene [[gene|hypO]] in response to disulfide stress

locus

BSU40540

BSU_40540

geneLength

375

378

function

control of the nitroreductase gene hypO in response to disulfide stress (diamide, NaOCl)

control of the nitroreductase gene [[gene|hypO]] in response to disulfide stress (diamide, NaOCl)

product

MarR/DUF24 family transcription regulator HypR

[SW|MarR family|MarR/DUF24 family] transcription regulator HypR

outlinks

bsu

BSU40540

BSU_40540

Gene

Coordinates

4,167,622 → 4,167,999

4,167,622 4,167,999

The protein

Protein family

[SW|MarR/DUF24 family]

[SW|MarR family|MarR/DUF24 family]

The protein

[SW|Domains]

5 alpha helices, 2 beta sheets, MarR-fold with wHTH motif, alpha4 major groove recognition helix, beta2 and 3 form the wing; alpha5 dimer interface [Pubmed|22238377]

[SW|HTH hxlR-type domain] (aa 14-112) (according to UniProt)

The protein

Modification

oxidized to Cys14-Cys49' intersubunit disulfides by disulfide stress [Pubmed|22238377]

Cys14 and Cys49' are about 8-9 Angström apart in reduced HypR-Dimer, oxidation moves the major groove recognition alpha4 helices of the HypR dimer about 4 Angstroem towards each other that leads to activation of HypR [Pubmed|22238377]

oxidized to Cys14-Cys49' intersubunit disulfides by disulfide stress [Pubmed|22238377]

Cys14 and Cys49' are about 8-9 Angstrm apart in reduced HypR-Dimer, oxidation moves the major groove recognition alpha4 helices of the HypR dimer about 4 Angstroem towards each other that leads to activation of HypR [Pubmed|22238377]

Biological materials

Mutant

MGNA-B836 (yybR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1835 NBRP B. subtilis, Japan]

BKE40540 (Δ[[gene|hypR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTGACCCTCCAATAG, downstream forward: _UP4_TAGCAGCAAAGGGAACTCCT

BKK40540 (Δ[[gene|hypR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTGACCCTCCAATAG, downstream forward: _UP4_TAGCAGCAAAGGGAACTCCT

MGNA-B836 (yybR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1835 NBRP B. subtilis, Japan]

BKE40540 ([[gene|hypR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTGACCCTCCAATAG, downstream forward: _UP4_TAGCAGCAAAGGGAACTCCT

BKK40540 ([[gene|hypR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTGACCCTCCAATAG, downstream forward: _UP4_TAGCAGCAAAGGGAACTCCT

Labs working on this gene/protein

[SW|Haike Antelmann],University of Greifswald, Germany

labs

[SW|Haike Antelmann],University of Greifswald, Germany