SubtiBank SubtiBank
Version comparison:

2017-8-11 16:0:32025-05-14 06:24:23

title

ykuL

darB

_ec

description

unknown

c-di-AMP binding protein

locus

BSU14130

BSU_14130

geneLength

441

444

product

unknown

c-di-AMP binding protein

names

ykuL

darB

ykuL

outlinks

bsu

BSU14130

BSU_14130

Gene

Coordinates

1,485,453 → 1,485,896

1,485,453 1,485,896

The protein

[SW|Domains]

[SW|CBS domain] (18-78)

[SW|CBS domain] (aa 18-78) (according to UniProt)

Biological materials

Mutant

MGNA-A773 (ykuL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/773 NBRP B. subtilis, Japan]

BKE14130 (''ykuL''::''erm trpC2'') available in the Bacillus Genetic Stock Center and in [SW|Jörg Stülke]'s lab [pubmed|28189581]

GP214 (deletion of ''[[gene|ykuJ]]-[[gene|ykuK]]-[[gene|abbA]]-[[gene|darB]]'', replaced by ''aphA3''), available in the [SW|Stülke] lab

MGNA-A773 (ykuL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/773 NBRP B. subtilis, Japan]

GP2769 (''Δ''''[[gene|darB]]''::''ermC''), available in [SW|Jörg Stülke]'s lab

BKE14130 (''ykuL''::''erm trpC2'') available in the Bacillus Genetic Stock Center and in [SW|Jörg Stülke]'s lab [pubmed|28189581]

GP214 (deletion of ''[[gene|ykuJ]]-[[gene|ykuK]]-[[gene|abbA]]-[[gene|darB]]'', replaced by ''aphA3''), available in [SW|Jörg Stülke]'s lab

BKE14130 ([[gene|darB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTCGACCCCTTTTC, downstream forward: _UP4_TAGGCTGTACGGTCCTATTT

BKK14130 ([[gene|darB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTCGACCCCTTTTC, downstream forward: _UP4_TAGGCTGTACGGTCCTATTT

GP2800 (''Δ''''[[gene|darB]]-[[gene|ccpC]]]''::''phleo''), available in [SW|Jörg Stülke]'s lab

Biological materials

Expression vector

pGP2929 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

References

14651647, 16395550

The protein

Paralogous protein(s)

[[this]]

The protein

Effectors of protein activity

binds c-di-AMP [pubmed|31061098]

Biological materials

Expression vectors

pGP2929 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP635: expression of Strep-''ykuL'' by [SW|pGP380] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

pGP767: expression of ''ykuL''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

pGP3306: expression of ''ykuL'' by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

Biological materials

two-hybrid system

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

Biological materials

FLAG-tag construct

GP2810 ''ykuL-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

labs

[SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]

References

Reviews

32603625

References

Original publications

14651647, 16395550, 31061098, 25215494