2017-8-11 16:0:32025-05-14 06:24:23
title
ykuL
darB
_ec
description
unknown
c-di-AMP binding protein
locus
BSU14130
BSU_14130
geneLength
441
444
product
unknown
c-di-AMP binding protein
names
ykuL
darB
ykuL
outlinks
bsu
BSU14130
BSU_14130
Gene
Coordinates
1,485,453 → 1,485,896
1,485,453 1,485,896
The protein
[SW|Domains]
[SW|CBS domain] (18-78)
[SW|CBS domain] (aa 18-78) (according to UniProt)
Biological materials
Mutant
MGNA-A773 (ykuL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/773 NBRP B. subtilis, Japan]
BKE14130 (''ykuL''::''erm trpC2'') available in the Bacillus Genetic Stock Center and in [SW|Jörg Stülke]'s lab [pubmed|28189581]
GP214 (deletion of ''[[gene|ykuJ]]-[[gene|ykuK]]-[[gene|abbA]]-[[gene|darB]]'', replaced by ''aphA3''), available in the [SW|Stülke] lab
MGNA-A773 (ykuL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/773 NBRP B. subtilis, Japan]
GP2769 (''Δ''''[[gene|darB]]''::''ermC''), available in [SW|Jörg Stülke]'s lab
BKE14130 (''ykuL''::''erm trpC2'') available in the Bacillus Genetic Stock Center and in [SW|Jörg Stülke]'s lab [pubmed|28189581]
GP214 (deletion of ''[[gene|ykuJ]]-[[gene|ykuK]]-[[gene|abbA]]-[[gene|darB]]'', replaced by ''aphA3''), available in [SW|Jörg Stülke]'s lab
BKE14130 ([[gene|darB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTCGACCCCTTTTC, downstream forward: _UP4_TAGGCTGTACGGTCCTATTT
BKK14130 ([[gene|darB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTCGACCCCTTTTC, downstream forward: _UP4_TAGGCTGTACGGTCCTATTT
GP2800 (''Δ''''[[gene|darB]]-[[gene|ccpC]]]''::''phleo''), available in [SW|Jörg Stülke]'s lab
Biological materials
Expression vector
pGP2929 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
References
The protein
Paralogous protein(s)
[[this]]
The protein
Effectors of protein activity
binds c-di-AMP [pubmed|31061098]
Biological materials
Expression vectors
pGP2929 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
pGP635: expression of Strep-''ykuL'' by [SW|pGP380] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
pGP767: expression of ''ykuL''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
pGP3306: expression of ''ykuL'' by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab
Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
Biological materials
FLAG-tag construct
GP2810 ''ykuL-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
labs
[SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]
References
Reviews
References
Original publications