Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)2025-05-13 11:10:29
description
transcription repressor of class III heat shock genes (clpC operon, clpE, clpP)
transcription repressor of class III heat shock genes ([[gene|clpC]] operon, [[gene|clpE]], [[gene|clpP]])
locus
BSU00830
BSU_00830
ec
outlinks
bsu
BSU00830
BSU_00830
aminos
MGHNISDIIEQYLKRVLDQNGKEILEIKRSEIADKFQCVPSQINYVINTRFTSERGYIVESKRGGGGYIRIIKIKMNNEVVLINNIISQINTHLSQAASDDIILRLLEDKVISEREAKMMVSVMDRSVLHIDLPERDELRARMMKAMLTSLKLK
[SW|Categories] containing this gene/protein
[SW|proteolysis], [SW|transcription factors and their control], [SW|general stress proteins (controlled by SigB)], [SW|heat shock proteins], [SW|phosphoproteins]
Gene
Coordinates on the chromosome (coding sequence)
101,449 -> 101,913
The protein
Protein family
ctsR family (according to Swiss-Prot)
CtsR family (single member, according to UniProt)
The protein
Modification
phosphorylated on Arg-55 (phosphorylation serves as tag for degradation by [SW|ClpC]-[SW|ClpP]) [Pubmed|27749819]
phosphorylation of a tyrosine residue by [[protein|McsB]] [Pubmed|14984053]
recently, it was reported that CtsR is phosphorylated by [[protein|McsB]] on Arg-62 rather than on a tyrosine residue [Pubmed|19498169]
in addition, Arg-15 was reported to be a phosphorylation site [Pubmed|22517742]
phosphorylated on Arg-55 (phosphorylation serves as tag for degradation by [[protein|ClpC]]-[[protein|ClpP]]) [Pubmed|27749819]
phosphorylation of a tyrosine residue by [[protein|McsB]] [Pubmed|14984053]
recently, it was reported that CtsR is phosphorylated by [[protein|McsB]] on Arg-62 rather than on a tyrosine residue [Pubmed|19498169]
in addition, Arg-15 was reported to be a phosphorylation site [Pubmed|22517742]
The protein
Structure
[PDB|3H0D] (complex with a 26bp DNA duplex, from ''Geobacillus stearothermophilus'') [Pubmed|19498169]
[PDB|3H0D] (complex with a 26bp DNA duplex, from ''Geobacillus stearothermophilus'') [Pubmed|19498169]
[PDB|6FH4] (C-terminal domain bound to phosphoarginine) [pubmed|30962626]
The protein
[SW|Interactions]
[[protein|McsA]]-[[protein|McsB]]-[[protein|CtsR]]
[[protein|CtsR]]-[[protein|ClpP]]/[[protein|ClpC]] (degradation of [[protein|CtsR]]) [Pubmed|11179229,16163393,17380125]
Expression and Regulation
Operon
''[[protein|ctsR]]-[[protein|mcsA]]-[[protein|mcsB]]-[[protein|clpC]]-[[protein|radA]]-[[protein|disA]]'' [Pubmed|8793870]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|8793870], [[protein|SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed1] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed2]
Expression and Regulation
Regulation
induced by stress ([[protein|SigB]]) [Pubmed|11544224]
repressed at low temperatures ([[protein|CtsR]]) [Pubmed|9987115]
Expression and Regulation
Regulatory mechanism
[[protein|CtsR]]: transcription repression [Pubmed|9987115]
Expression and Regulation
Additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
the mRNA is very stable (> 15 min) [pubmed|12884008]
Biological materials
Mutant
''ctsR::aphA3'' availbale in [SW|Ulf Gerth]'s lab
BKE00830 (''[[protein|ctsR]]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab)
GP3202([[gene|ctsR]]::''ermC''), available in [SW|Jörg Stülke]'s lab
MGNA-B928 (yacG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1927 NBRP B. subtilis, Japan]
''ctsR::aphA3'' availbale in [SW|Ulf Gerth]'s lab
BKE00830 (''[[gene|ctsR]]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
BKE00830 ([[gene|ctsR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCAACCCCCTCCTTTAC, downstream forward: _UP4_AAATTAAAATAAGCGGGTGA
BKK00830 ([[gene|ctsR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCAACCCCCTCCTTTAC, downstream forward: _UP4_AAATTAAAATAAGCGGGTGA
Biological materials
Expression vector
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab
References
Reviews
References
Original Publications
proteinLength
154
geneLength
465
Gene
Coordinates
101,449 101,913
Gene
Phenotypes of a mutant
reduced expression of [[gene|trxB]], due to reduced [[protein|Spx]] activity [pubmed|30962353]
The protein
Paralogous protein(s)
[[this]]
The protein
additional information
subject to Clp-dependent proteolysis upon glucose starvation [pubmed|17981983]
information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000017 PRODORIC2 database]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab
Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab