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Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)2025-05-13 11:10:29

description

transcription repressor of class III heat shock genes (clpC operon, clpE, clpP)

transcription repressor of class III heat shock genes ([[gene|clpC]] operon, [[gene|clpE]], [[gene|clpP]])

locus

BSU00830

BSU_00830

ec

outlinks

bsu

BSU00830

BSU_00830

aminos

MGHNISDIIEQYLKRVLDQNGKEILEIKRSEIADKFQCVPSQINYVINTRFTSERGYIVESKRGGGGYIRIIKIKMNNEVVLINNIISQINTHLSQAASDDIILRLLEDKVISEREAKMMVSVMDRSVLHIDLPERDELRARMMKAMLTSLKLK

[SW|Categories] containing this gene/protein

[SW|proteolysis], [SW|transcription factors and their control], [SW|general stress proteins (controlled by SigB)], [SW|heat shock proteins], [SW|phosphoproteins]

Gene

Coordinates on the chromosome (coding sequence)

101,449 -> 101,913

The protein

Protein family

ctsR family (according to Swiss-Prot)

CtsR family (single member, according to UniProt)

The protein

Modification

phosphorylated on Arg-55 (phosphorylation serves as tag for degradation by [SW|ClpC]-[SW|ClpP]) [Pubmed|27749819]

phosphorylation of a tyrosine residue by [[protein|McsB]] [Pubmed|14984053]

recently, it was reported that CtsR is phosphorylated by [[protein|McsB]] on Arg-62 rather than on a tyrosine residue [Pubmed|19498169]

in addition, Arg-15 was reported to be a phosphorylation site [Pubmed|22517742]

phosphorylated on Arg-55 (phosphorylation serves as tag for degradation by [[protein|ClpC]]-[[protein|ClpP]]) [Pubmed|27749819]

phosphorylation of a tyrosine residue by [[protein|McsB]] [Pubmed|14984053]

recently, it was reported that CtsR is phosphorylated by [[protein|McsB]] on Arg-62 rather than on a tyrosine residue [Pubmed|19498169]

in addition, Arg-15 was reported to be a phosphorylation site [Pubmed|22517742]

The protein

Structure

[PDB|3H0D] (complex with a 26bp DNA duplex, from ''Geobacillus stearothermophilus'') [Pubmed|19498169]

[PDB|3H0D] (complex with a 26bp DNA duplex, from ''Geobacillus stearothermophilus'') [Pubmed|19498169]

[PDB|6FH4] (C-terminal domain bound to phosphoarginine) [pubmed|30962626]

The protein

[SW|Interactions]

[[protein|McsA]]-[[protein|McsB]]-[[protein|CtsR]]

[[protein|CtsR]]-[[protein|ClpP]]/[[protein|ClpC]] (degradation of [[protein|CtsR]]) [Pubmed|11179229,16163393,17380125]

Expression and Regulation

Operon

''[[protein|ctsR]]-[[protein|mcsA]]-[[protein|mcsB]]-[[protein|clpC]]-[[protein|radA]]-[[protein|disA]]'' [Pubmed|8793870]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|8793870], [[protein|SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed1] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed2]

Expression and Regulation

Regulation

induced by stress ([[protein|SigB]]) [Pubmed|11544224]

repressed at low temperatures ([[protein|CtsR]]) [Pubmed|9987115]

Expression and Regulation

Regulatory mechanism

[[protein|CtsR]]: transcription repression [Pubmed|9987115]

Expression and Regulation

Additional information

the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

the mRNA is very stable (> 15 min) [pubmed|12884008]

Biological materials

Mutant

''ctsR::aphA3'' availbale in [SW|Ulf Gerth]'s lab

BKE00830 (''[[protein|ctsR]]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab)

GP3202([[gene|ctsR]]::''ermC''), available in [SW|Jörg Stülke]'s lab

MGNA-B928 (yacG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1927 NBRP B. subtilis, Japan]

''ctsR::aphA3'' availbale in [SW|Ulf Gerth]'s lab

BKE00830 (''[[gene|ctsR]]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]

BKE00830 ([[gene|ctsR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCAACCCCCTCCTTTAC, downstream forward: _UP4_AAATTAAAATAAGCGGGTGA

BKK00830 ([[gene|ctsR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCAACCCCCTCCTTTAC, downstream forward: _UP4_AAATTAAAATAAGCGGGTGA

Biological materials

Expression vector

for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab

References

Reviews

27518094

14984053, 21078442, 23375660, 27518094, 28402413, 28748186

References

Original Publications

14984053, 21078442, 23375660, 27749819

27749819, 30962626, 30962353, 11179229, 20852588

proteinLength

154

geneLength

465

Gene

Coordinates

101,449 101,913

Gene

Phenotypes of a mutant

reduced expression of [[gene|trxB]], due to reduced [[protein|Spx]] activity [pubmed|30962353]

The protein

Paralogous protein(s)

[[this]]

The protein

additional information

subject to Clp-dependent proteolysis upon glucose starvation [pubmed|17981983]

information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000017 PRODORIC2 database]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Expression vectors

for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab

Biological materials

two-hybrid system

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab