SubtiBank SubtiBank
Version comparison:

Wed Feb 15 2017 17:48:01 GMT+0100 (CET)2025-05-09 00:58:24

locus

BSU30190

BSU_30190

ec

outlinks

bsu

BSU30190

BSU_30190

[SW|Categories] containing this gene/protein

[SW|biosynthesis of cofactors]

Gene

Coordinates on the chromosome (coding sequence)

3,089,226 -> 3,090,413

The protein

Catalyzed reaction/ biological activity

formation of pimelic acid via a multiple-step oxidative cleavage of long-chain fatty acids

formation of pimelic acid via a multiple-step oxidative cleavage of long-chain fatty acids

C2-C8-saturated long-chain fatty acyl-[ACP] + 3 O2 + 2 reduced [flavodoxin] --> fatty aldehyde + 3 H+ + 3 H2O + 2 oxidized [flavodoxin] + pimeloyl-[ACP] (according to UniProt)

The protein

Protein family

[SW|cytochrome P450] family (according to Swiss-Prot)

[SW|cytochrome P450] family (according to UniProt)

The protein

[SW|Interactions]

[[protein|Fer]]-[[protein|BioI]] [Pubmed|12538057]

[[protein|YkuP]]-[[protein|BioI]] [Pubmed|15449930]

[[protein|AcpA]]-[[protein|BioI]] [Pubmed|18838690]

Expression and Regulation

Operon

''[[protein|bioW]]-[[protein|bioA]]-[[protein|bioF]]-[[protein|bioD]]-[[protein|bioB]]-[[protein|bioI]]-[[protein|ytbQ]]'' [Pubmed|8763940,8892842]

Expression and Regulation

Regulation

repressed in the presence of biotin ([[protein|BirA]]) [Pubmed|8763940,8892842]

Expression and Regulation

Regulatory mechanism

[[protein|BirA]]: transcription repression [Pubmed|8763940,8892842]

Biological materials

Mutant

MGNA-A804 (bioI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/804 NBRP B. subtilis, Japan]

MGNA-A804 (bioI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/804 NBRP B. subtilis, Japan]

BKE30190 ([[gene|bioI]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTGATTTTCTCCTTTCT, downstream forward: _UP4_TAAGCCTAAGAATGTGAGTG

BKK30190 ([[gene|bioI]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTGATTTTCTCCTTTCT, downstream forward: _UP4_TAAGCCTAAGAATGTGAGTG

References

Reviews

[Pubmed|21437340]==

21437340

Original publications

18838690, 8763940, 8892842, 15449931, 15449930, 12538057, 20658980, 11368323, 11472016, 14737344, 12943422, 28196402

proteinLength

395

geneLength

1188

Gene

Coordinates

3,089,226 3,090,413

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

References

Original publications

18838690, 8763940, 8892842, 15449931, 15449930, 12538057, 20658980, 11368323, 11472016, 14737344, 12943422, 28196402, 28898812