SubtiBank SubtiBank
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Mon Oct 31 2016 11:08:56 GMT+0100 (CET)2025-05-13 17:55:23

description

acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4), coordinates membrane synthesis with cell division

acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4)

locus

BSU15890

BSU_15890

outlinks

bsu

BSU15890

BSU_15890

[SW|Categories] containing this gene/protein

[SW|cell division], [SW|biosynthesis of lipids], [SW|essential genes]

Gene

Coordinates on the chromosome (coding sequence)

1,662,547 -> 1,663,548

Gene

Phenotypes of a mutant

essential [Pubmed|24224907,12682299]

depletion results in sporulation deficiency (via [SW|SpoIIR]) [Pubmed|22111992]

a ts mutant forms an abberant Z-ring and has a [SW|cell division] defect [Pubmed|24224907]

essential [Pubmed|24224907,12682299]

depletion results in sporulation deficiency (via [[protein|SpoIIR]]) [Pubmed|22111992]

a ts mutant forms an abberant Z-ring and has a [SW|cell division] defect [Pubmed|24224907]

depletion results in accumulation of malonyl-CoA and of long-chain-ACPs [pubmed|32671874]

The protein

Catalyzed reaction/ biological activity

catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4)

catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4)

fatty acyl-[ACP] + phosphate --> acyl phosphate + holo-[ACP] (according to UniProt)

The protein

Protein family

plsX family (according to Swiss-Prot)

plsX family (single member, according to UniProt)

The protein

Structure

[PDB|1VI1] [Pubmed|16021622]

[PDB|1VI1] [Pubmed|16021622]

[PDB|6A1K] [pubmed|31453380]

The protein

[SW|Localization]

localizes to the cell division sites, colocalizes with the Z-ring [Pubmed|24224907]

uniformly distributed on the membrane of most cells, but occasionally appears as membrane foci as well [Pubmed|26816052]

in the presence of daptomycin, PlsX delocalizes from the membrane to the cytoplasm [Pubmed|27791134]

localizes to regions of increased fluidity in the membrane (via a short amphipathic α-peptide) [pubmed|31453380,31925177]

uniformly distributed on the membrane of most cells, but occasionally appears as membrane foci as well [Pubmed|26816052]

in the presence of daptomycin, PlsX delocalizes from the membrane to the cytoplasm [Pubmed|27791134]

The protein

[SW|Interactions]

[[protein|PlsX]]-[[protein|PlsY]] [Pubmed|19282621]

[[protein|PlsX]]-[[protein|FtsA]] [Pubmed|24224907]

Expression and Regulation

Operon

''[[protein|fapR]]-[[protein|plsX]]-[[protein|fabD]]-[[protein|fabG]]-[[protein|acpA]]'' [Pubmed|12737802,19850612]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|16932747]

Expression and Regulation

Regulation

induced if the cells experience an accumulation of malonyl-CoA ([[protein|FapR]]) [Pubmed|12737802]

expressed at high cell density ([[protein|ComA]]) [Pubmed|16091051]

induced upon fatty acid biosynthesis inhibition [Pubmed|21383089]

Expression and Regulation

Regulatory mechanism

[[protein|ComA]]: transcription activation ([[protein|ComA]]) [Pubmed|16091051]

[[protein|FapR]]: transcription repression [Pubmed|12737802]

References

12737802, 19282621, 17645809, 17557823, 8759840, 16932747, 19850612, 16091051, 21383089, 22111992, 24224907, 26816052, 16021622, 27791134

12737802, 19282621, 17645809, 17557823, 8759840, 16932747, 19850612, 16091051, 21383089, 22111992, 24224907, 26816052, 16021622, 27791134, 31453380, 31925177, 31919098, 32180316, 32671874

proteinLength

333

geneLength

1002

Gene

Coordinates

1,662,547 1,663,548

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]